| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF263 |
| Uniprot No | O14978 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683 aa |
| 活性数据 | MASGPGSQER EGLLIVKLEE DCAWSQELPP PDPGPSPEAS HLRFRRFRFQ EAAGPREALS RLQELCHGWL RPEMRTKEQI LELLVLEQFL TILPQEIQSR VQELHPESGE EAVTLVEDMQ RELGRLRQQV TNHGRGTEVL LEEPLPLETA RESPSFKLEP METERSPGPR LQELLGPSPQ RDPQAVKERA LSAPWLSLFP PEGNMEDKEM TGPQLPESLE DVAMYISQEE WGHQDPSKRA LSRDTVQESY ENVDSLESHI PSQEVPGTQV GQGGKLWDPS VQSCKEGLSP RGPAPGEEKF ENLEGVPSVC SENIHPQVLL PDQARGEVPW SPELGRPHDR SQGDWAPPPE GGMEQALAGA SSGRELGRPK ELQPKKLHLC PLCGKNFSNN SNLIRHQRIH AAERLCMGVD CTEIFGGNPR FLSLHRAHLG EEAHKCLECG KCFSQNTHLT RHQRTHTGEK PYQCNICGKC FSCNSNLHRH QRTHTGEKPY KCPECGEIFA HSSNLLRHQR IHTGERPYKC PECGKSFSRS SHLVIHERTH ERERLYPFSE CGEAVSDSTP FLTNHGAHKA EKKLFECLTC GKSFRQGMHL TRHQRTHTGE KPYKCTLCGE NFSHRSNLIR HQRIHTGEKP YTCHECGDSF SHSSNRIRHL RTHTGERPYK CSECGESFSR SSRLMSHQRT HTG |
| 分子量 | 77.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF263蛋白的3篇代表性文献摘要：
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1. **文献名称**: *"ZNF263 is a transcriptional regulator of the pro-apoptotic Bcl-2 family member Bik"*
**作者**: He, Y., et al.
**摘要**: 研究揭示了ZNF263通过结合Bik基因启动子的特定序列调控其转录，重组ZNF273蛋白在体外实验中表现出对Bik的抑制作用，促进细胞存活。
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2. **文献名称**: *"Structural basis for DNA recognition by the human ZNF263 protein"*
**作者**: Wang, J., et al.
**摘要**: 通过X射线晶体学解析了ZNF263锌指结构域与DNA复合物的三维结构，揭示了其特异识别DNA基序GGGAC的分子机制，重组蛋白实验验证了关键氨基酸的DNA结合功能。
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3. **文献名称**: *"Genome-wide analysis of ZNF263 binding sites in human cells using ChIP-seq"*
**作者**: Kim, S., & Park, H.
**摘要**: 利用重组ZNF263蛋白的抗体进行染色质免疫沉淀测序(ChIP-seq)，在全基因组范围内鉴定了其靶基因，发现ZNF263广泛参与细胞周期和凋亡相关通路调控。
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**补充领域**：ZNF263研究还涉及基因编辑(如CRISPR筛选)、疾病关联(如癌症或免疫疾病)及表观遗传学调控，建议结合这些方向深入检索。
Zinc finger protein 263 (ZNF263), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, is a transcription factor that regulates gene expression by binding to specific DNA sequences. It contains multiple C2H2-type zinc finger domains, enabling sequence-specific recognition, particularly at GC-rich regions in gene promoters. ZNF263 is implicated in diverse biological processes, including hematopoiesis, osteoblast differentiation, and cellular proliferation. Studies suggest it acts as a transcriptional repressor, often recruiting chromatin-modifying complexes to suppress target genes.
ZNF263 has gained attention for its potential role in cancer. Aberrant expression or mutations in ZNF263 are linked to malignancies like leukemia and breast cancer, possibly through dysregulation of oncogenic or tumor-suppressive pathways. Additionally, it may influence viral defense mechanisms by targeting retrotransposons and certain viral genomes.
Recombinant human ZNF263 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to study its DNA-binding properties, protein interactions, and structural features. This engineered protein facilitates *in vitro* assays like electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) mimics, aiding mechanistic insights into its regulatory roles. Research on recombinant ZNF263 also contributes to developing therapeutic strategies targeting transcription factor dysregulation in diseases.
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