| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF266 |
| Uniprot No | Q14584 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549 aa |
| 活性数据 | MLENYKNLAT VGYQLFKPSL ISWLEQEESR TVQRGDFQAS EWKVQLKTKE LALQQDVLGE PTSSGIQMIG SHNGGEVSDV KQCGDVSSEH SCLKTHVRTQ NSENTFECYL YGVDFLTLHK KTSTGEQRSV FSQCGKAFSL NPDVVCQRTC TGEKAFDCSD SGKSFINHSH LQGHLRTHNG ESLHEWKECG RGFIHSTDLA VRIQTHRSEK PYKCKECGKG FRYSAYLNIH MGTHTGDNPY ECKECGKAFT RSCQLTQHRK THTGEKPYKC KDCGRAFTVS SCLSQHMKIH VGEKPYECKE CGIAFTRSSQ LTEHLKTHTA KDPFECKICG KSFRNSSCLS DHFRIHTGIK PYKCKDCGKA FTQNSDLTKH ARTHSGERPY ECKECGKAFA RSSRLSEHTR THTGEKPFEC VKCGKAFAIS SNLSGHLRIH TGEKPFECLE CGKAFTHSSS LNNHMRTHSA KKPFTCMECG KAFKFPTCVN LHMRIHTGEK PYKCKQCGKS FSYSNSFQLH ERTHTGEKPY ECKECGKAFS SSSSFRNHER RHADERLSA |
| 分子量 | 62.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF266蛋白的三篇参考文献示例，涵盖表达、功能及疾病相关研究：
1. **文献名称**：*Cloning and Characterization of Human ZNF266: A Novel Kruppel-Associated Box-Containing Zinc Finger Protein*
**作者**：Zhang, Y. et al.
**摘要**：该研究报道了ZNF266的全长cDNA克隆，并通过原核表达系统成功纯化重组蛋白。实验证实ZNF266具有DNA结合活性，其KRAB结构域可能参与转录调控，为后续功能研究奠定基础。
2. **文献名称**：*Structural and Functional Analysis of ZNF266 in Transcriptional Repression*
**作者**：Li, X. et al.
**摘要**：利用HEK293细胞表达重组ZNF266蛋白，发现其通过KRAB结构域招募组蛋白去乙酰化酶复合物(HDAC)，抑制下游基因转录。ChIP实验证实其在特定启动子区域的富集，提示表观遗传调控作用。
3. **文献名称**：*ZNF266 Interacts with Ubiquitin-Conjugating Enzymes and Modulates Protein Stability*
**作者**：Wang, T. et al.
**摘要**：通过Pull-down和质谱分析，鉴定ZNF266与E3泛素连接酶复合物相互作用。过表达重组ZNF266可促进靶蛋白泛素化降解，暗示其在细胞周期或癌症发生中的潜在调节功能。
以上文献内容覆盖重组ZNF266的分子特性、结构功能及疾病关联，均为假设性示例，实际研究需查询权威数据库如PubMed获取准确信息。
Zinc finger protein 266 (ZNF266), also known as Krueppel-related zinc finger protein 7 (KR7), is a member of the Krueppel C2H2-type zinc finger protein family. These proteins are characterized by tandem arrays of zinc finger motifs, which typically function in sequence-specific DNA or RNA binding, facilitating roles in transcriptional regulation, chromatin remodeling, and protein interactions. ZNF266 contains multiple C2H2 zinc finger domains and is thought to participate in epigenetic regulation, possibly through interactions with chromatin-modifying complexes or by targeting specific gene promoters. Though its precise biological functions remain understudied, ZNF266 is expressed in various human tissues and has been implicated in neurological processes and tumorigenesis. For instance, altered expression patterns have been observed in certain cancers and neurodevelopmental disorders, suggesting potential roles in cellular differentiation and disease pathways. Recombinant human ZNF266 protein, produced via expression systems like E. coli or mammalian cells, enables in vitro studies to decipher its molecular interactions, DNA-binding specificity, and structural properties. Current research focuses on identifying its target genes and regulatory mechanisms, as well as exploring its diagnostic or therapeutic potential in diseases linked to zinc finger dysregulation. However, comprehensive functional insights remain limited, necessitating further biochemical and genomic investigations.
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