| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF302 |
| Uniprot No | Q9NR11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478 aa |
| 活性数据 | MSQVTFSDVA IDFSHEEWAC LDSAQRDLYK DVMVQNYENL VSVGLSVTKP YVIMLLEDGK EPWMMEKKLS KAYPFPLSHS VPASVNFGFS ALFEHCSEVT EIFELSELCV FWVLHFLSNS PNSTVEAFSR SKKKKKKKKK RQCFAFLIYF RLGIKMGKQG IINKEGYLYE DSPQPVTMEK VVKQSYEFSN SNKNLEYTEC DTFRSTFHSK STLSEPQNNS AEGNSHKYDI LKKNLSKKSV IKSERINGGK KLLNSNKSGA AFNQSKSLTL PQTCNREKIY TCSECGKAFG KQSILSRHWR IHTGEKPYEC RECGKTFSHG SSLTRHQISH SGEKPYKCIE CGKAFSHGSS LTNHQSTHTG EKPYECMNCG KSFSRVSLLI QHLRIHTQEK RYECRICGKA FIHSSSLIHH QKSHTGEKPY ECRECGKAFC CSSHLTQHQR IHSMKKKYEC NKCLKVFSSF SFLVQHQSIH TEEKPFEV |
| 分子量 | 54.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF302蛋白的3-4条示例参考文献格式(注：以下内容为虚构示例，实际文献需通过学术数据库查询)：
1. **《Characterization of human ZNF302 as a novel transcriptional repressor》**
- 作者：Li, X., et al.
- 摘要：本研究克隆并表达了重组人ZNF302蛋白，发现其通过C2H2型锌指结构域结合特定DNA序列，抑制下游靶基因的转录活性，提示其在表观遗传调控中的潜在作用。
2. **《ZNF302 overexpression correlates with poor prognosis in breast cancer》**
- 作者：Zhang, Y., et al.
- 摘要：通过免疫组化和重组蛋白功能实验，证明ZNF302在乳腺癌组织中高表达，并通过调控细胞周期相关基因促进肿瘤增殖和转移。
3. **《Structural basis of ZNF302-DNA interaction revealed by X-ray crystallography》**
- 作者：Wang, T., et al.
- 摘要：解析了ZNF302蛋白锌指结构域与DNA结合的晶体结构，揭示了其特异性识别的碱基序列模式，为开发靶向药物提供了结构基础。
4. **《ZNF302 regulates pluripotency in embryonic stem cells via epigenetic modifications》**
- 作者：Chen, L., et al.
- 摘要：利用重组ZNF302蛋白和基因编辑技术，证明其通过招募组蛋白修饰复合物维持胚胎干细胞多能性，影响关键发育基因的表达。
**建议**：实际研究中可查阅PubMed、Google Scholar等平台，使用关键词“ZNF302 protein”或“ZNF302 recombinant”获取真实文献。注意核对研究领域(如结构、癌症、干细胞等)以筛选相关论文。
ZNF302 is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that mediate sequence-specific DNA binding or protein-protein interactions. As a transcription regulator, it plays roles in gene expression modulation, though its exact biological functions remain less characterized compared to other zinc finger proteins. Studies suggest its involvement in cellular processes such as differentiation, proliferation, and apoptosis. Recombinant human ZNF302 protein is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for in vitro studies to dissect its molecular mechanisms. Interest in ZNF302 stems from its potential association with cancer progression and stem cell regulation. For example, it has been reported to repress tumor-suppressor genes in certain malignancies by binding promoter regions or interacting with epigenetic modifiers. Additionally, ZNF302 may participate in maintaining pluripotency in embryonic stem cells by silencing lineage-specific genes. Structural analyses reveal its multi-zinc finger architecture enables diverse nucleic acid targeting, but comprehensive interactome and chromatin-binding profiles are still under exploration. Current research focuses on clarifying its tissue-specific roles, post-translational modifications, and therapeutic relevance in diseases. The availability of recombinant ZNF302 facilitates biochemical assays, such as DNA-binding affinity tests and screening for small-molecule inhibitors.
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