| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF28 |
| Uniprot No | P17035 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718 aa |
| 活性数据 | MALPQGLLTF RDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLDIS SKCMMKTFFS TGQGNTEAFH TGTLQRQASH HIGDFCFQKI EKDIHGFQFQ WKEDETNDHA APMTEIKELT GSTGQHDQRH AGNKHIKDQL GLSFHSHLPE LHIFQPEGKI GNQVEKSINN ASSVSTSQRI CCRPKTHISN KYGNNSLHSS LLTQKRNVHM REKSFQCIES GKSFNCSSLL KKHQITHLEE KQCKCDVYGK VFNQKRYLAC HRRSHIDEKP YKCNECGKIF GHNTSLFLHK ALHTADKPYE CEECDKVFSR KSHLETHKII YTGGKPYKCK VCDKAFTCNS YLAKHTIIHT GEKPYKCNEC GKVFNRLSTL ARHRRLHTGE KPYECEECEK VFSRKSHLER HKRIHTGEKP YKCKVCDKAF AYNSYLAKHS IIHTGEKPYK CNECGKVFNQ QSTLARHHRL HTAEKPYKCE ECDKVFRCKS HLERHRRIHT GEKPYKCKVC DKAFRSDSCL TEHQRVHTGE KPYMCNECGK VFSTKANLAC HHKLHTAEKP YKCEECEKVF SRKSHMERHR RIHTGEKPYK CKVCDKAFRR DSHLAQHQRV HTGEKPYKCN ECGKTFRQTS SLIIHRRLHT GEKPYKCNEC GKTFSQMSSL VYHHRLHSGE KPYKCNECGK VFNQQAHLAQ HQRVHTGEKP YKCNECGKTF SQMSNLVYHH RLHSGEKP |
| 分子量 | 83.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
目前公开学术数据库中关于ZNF28蛋白的专项研究较为有限，以下为基于相关领域的模拟参考文献示例(建议通过权威数据库进一步验证)：
1. **"Structural characterization of the zinc finger protein ZNF28 and its DNA-binding properties"**
- Author: Smith J, et al. (2015)
- 摘要：通过重组表达纯化人ZNF28蛋白，解析其锌指结构域与特定DNA序列的结合模式，揭示了其在基因调控中的潜在作用。
2. **"ZNF28 overexpression modulates cell proliferation in human cancer cell lines"**
- Author: Lee S, et al. (2018)
- 摘要：研究重组ZNF28蛋白在肿瘤细胞中的功能，发现其过表达通过抑制Wnt/β-catenin通路显著降低细胞增殖能力。
3. **"Optimization of recombinant ZNF28 production in E. coli for functional studies"**
- Author: Gonzalez R, et al. (2020)
- 摘要：报道了在大肠杆菌系统中高效表达可溶性ZNF28蛋白的优化策略，并验证了纯化后蛋白的体外活性。
**备注**：以上为模拟参考，实际文献建议通过PubMed或Google Scholar以关键词“ZNF28”“recombinant ZNF28”检索最新研究。锌指蛋白家族研究(如ZNF类综述)中可能包含ZNF28的阶段性结论。
Zinc Finger Protein 28 (ZNF28), a member of the Krüppel-associated box (KRAB)-zinc finger (ZNF) protein family, is a transcription factor encoded by the *ZNF28* gene in humans. Zinc finger proteins are characterized by tandem arrays of zinc-binding domains that facilitate DNA, RNA, or protein interactions. The KRAB domain, located at the N-terminus, typically recruits co-repressor complexes to mediate transcriptional repression. ZNF28. like other KRAB-ZNFs, is postulated to regulate gene expression by binding to specific DNA sequences through its C-terminal zinc finger motifs, influencing chromatin structure and transcriptional activity.
While the exact biological roles of ZNF28 remain less characterized compared to other ZNFs, studies suggest it may participate in cell differentiation, proliferation, and oncogenesis. Recombinant human ZNF28 protein is engineered for experimental use, often expressed in bacterial or mammalian systems with affinity tags (e.g., His-tag) for purification. This recombinant form enables functional studies, such as DNA-binding assays, protein interaction analyses, and investigations into its regulatory mechanisms.
Despite its potential significance, limited data exist on ZNF28's specific targets or pathways. Ongoing research aims to elucidate its role in development and disease, leveraging recombinant tools to explore its interactions within transcriptional networks. As part of the largest transcription factor family in mammals, ZNF28 underscores the functional diversity of KRAB-ZNF proteins in genome regulation and cellular homeostasis.
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