| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF285 |
| Uniprot No | Q96NJ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-590 aa |
| 活性数据 | MIKFQERVTF KDVAVVFTKE ELALLDKAQI NLYQDVMLEN FRNLMLVRDG IKNNILNLQA KGLSYLSQEV LHCWQIWKQR IRDLTVSQDY IVNLQEECSP HLEDVSLSEE WAGISLQISE NENYVVNAII KNQDITAWQS LTQVLTPESW RKANIMTEPQ NSQGRYKGIY MEEKLYRRAQ HDDSLSWTSC DHHESQECKG EDPGRHPNCG KNLGMKSTVE KRNAAHVLPQ PFPCNNCGVA FADDTDPHVH HSTHLGEKSY KCDQYGKNFS QSQDLIVHCK THSGKTPYEF HEWPMGCKQS SDLPRYQKVS SGDKPYKCKE CGKGFRRSSS LHNHHRVHTG EMPYKCDECG KGFGFRSLLC IHQGVHTGKK PYKCEECGKG FDQSSNLLVH QRVHTGEKPY KCSECGKCFS SSSVLQVHWR FHTGEKPYRC GECGKGFSQC THLHIHQRVH TGEKPYKCNV CGKDFAYSSV LHTHQRVHTG EKPYKCEVCG KCFSYSSYFH LHQRDHIREK PYKCDECGKG FSRNSDLNVH LRVHTRERPY KCKACGKGFS RNSYLLAHQR VHIDETQYTH CERGKDLLTH QRLHEQRETL |
| 分子量 | 68.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF285蛋白的参考文献示例(注：以下文献为假设性示例，实际引用时请核实真实文献)：
1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human ZNF285 Protein*
**作者**：Zhang Y. et al.
**摘要**：本研究成功克隆并表达了重组人ZNF285蛋白，利用HEK293细胞系统进行纯化，发现其锌指结构域能够特异性结合特定DNA序列，提示其在基因转录调控中的潜在作用。
2. **文献名称**：*ZNF285 Overexpression Alters Cell Proliferation in Breast Cancer Models*
**作者**：Li X. et al.
**摘要**：通过重组ZNF285蛋白的功能实验，发现其在乳腺癌细胞中通过调控细胞周期相关基因(如CDKN1A)抑制增殖，提示其可能作为肿瘤抑制因子。
3. **文献名称**：*Structural Analysis of ZNF285 Reveals Key Domains for Protein-Protein Interaction*
**作者**：Wang J. et al.
**摘要**：通过X射线晶体学解析了重组ZNF285蛋白的三维结构，揭示了其N端锌指结构域与C端非典型结构域在介导蛋白质相互作用中的关键功能。
4. **文献名称**：*ZNF285 Modulates Wnt/β-catenin Signaling via Recombinant Protein Interaction Assays*
**作者**：Chen L. et al.
**摘要**：利用重组ZNF285蛋白的体外结合实验，证实其通过结合β-catenin抑制Wnt信号通路活性，为癌症靶向治疗提供新靶点。
**注意**：以上为模拟文献，实际研究中关于ZNF285的重组蛋白文献较少。建议在PubMed或Web of Science中使用关键词“ZNF285 recombinant”或“ZNF285 protein function”进一步检索真实数据。
Zinc finger protein 285 (ZNF285), a member of the Krüppel-associated box (KRAB) domain zinc finger protein family, is a transcription factor encoded by the human ZNF285 gene located on chromosome 19q13.43. Characterized by its C2H2-type zinc finger motifs, ZNF285 binds to specific DNA sequences, regulating gene expression through transcriptional activation or repression. It is implicated in developmental processes, cellular differentiation, and tumorigenesis, though its exact biological roles remain under investigation. Recombinant human ZNF285 protein is artificially expressed in vitro using systems like Escherichia coli or mammalian cell cultures, enabling functional studies in molecular biology. Researchers utilize this engineered protein to explore its DNA-binding specificity, interaction partners, and regulatory mechanisms in cellular pathways. Emerging evidence links ZNF285 dysregulation to certain cancers, including breast and ovarian malignancies, where aberrant expression patterns correlate with clinical outcomes. However, comprehensive mechanistic insights and disease-specific targets require further validation. Current research focuses on elucidating its epigenetic regulatory functions and potential as a biomarker or therapeutic target, leveraging recombinant protein tools for structural analysis and high-throughput screening assays. The availability of recombinant ZNF285 facilitates biochemical characterization and advances understanding of zinc finger protein-mediated gene networks in health and disease.
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