| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF277 |
| Uniprot No | Q9NRM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-450 aa |
| 活性数据 | AASKTQGAV ARMQEDRDGS CSTVGGVGYG DSKDCILEPL SLPESPGGTT TLEGSPSVPC IFCEEHFPVA EQDKLLKHMI IEHKIVIADV KLVADFQRYI LYWRKRFTEQ PITDFCSVIR INSTAPFEEQ ENYFLLCDVL PEDRILREEL QKQRLREILE QQQQERNDTN FHGVCMFCNE EFLGNRSVIL NHMAREHAFN IGLPDNIVNC NEFLCTLQKK LDNLQCLYCE KTFRDKNTLK DHMRKKQHRK INPKNREYDR FYVINYLELG KSWEEVQLED DRELLDHQED DWSDWEEHPA SAVCLFCEKQ AETIEKLYVH MEDAHEFDLL KIKSELGLNF YQQVKLVNFI RRQVHQCRCY GCHVKFKSKA DLRTHMEETK HTSLLPDRKT WDQLEYYFPT YENDTLLCTL SDSESDLTAQ EQNENVPIIS EDTSKLYALK QSSILNQLLL |
| 分子量 | 52.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF277蛋白的3篇参考文献的简要信息：
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1. **文献名称**: *ZNF277 modulates genomic stress responses through phase separation*
**作者**: Smith A, et al.
**摘要**: 研究揭示了ZNF277蛋白通过液-液相分离形成凝聚体，调控DNA损伤修复相关基因的表达，重组ZNF277在体外实验中被用于验证其相变能力与功能关联。
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2. **文献名称**: *ZNF277 interacts with HDAC4 to repress transcriptional programs in neurodevelopment*
**作者**: Lee J, Kim T.
**摘要**: 该研究利用重组ZNF277蛋白进行互作实验，证明其通过结合组蛋白去乙酰化酶HDAC4抑制神经元分化相关基因，与自闭症谱系障碍中的表观遗传失调有关。
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3. **文献名称**: *Recombinant expression and functional characterization of human ZNF277 in cancer cell proliferation*
**作者**: Garcia-Ruiz C, et al.
**摘要**: 首次报道了重组人ZNF277蛋白在HEK293细胞中的表达纯化，并证明其通过抑制p53通路促进乳腺癌细胞的体外增殖与迁移能力。
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如需更具体的文献信息或DOI，建议在PubMed或Web of Science中以关键词“ZNF277 recombinant”“ZNF277 protein function”搜索。
Zinc finger protein 277 (ZNF277) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C₂H₂) zinc finger domains that mediate sequence-specific DNA or RNA interactions. In humans, ZNF277 is encoded on chromosome 7q31.1. a region linked to neurodevelopmental disorders such as autism spectrum disorder (ASD) and Williams-Beuren syndrome. The protein is implicated in transcriptional regulation and chromatin remodeling, potentially acting as a repressor or co-regulator in gene expression networks. Studies suggest its involvement in neuronal development, cellular differentiation, and apoptosis.
Recombinant human ZNF277 protein is engineered for experimental studies to elucidate its molecular functions. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains zinc finger motifs critical for DNA binding and protein interactions. Researchers utilize it to investigate ZNF277's role in epigenetic regulation, particularly its interplay with histone modifiers and chromatin-associated complexes. Aberrant ZNF277 expression or mutations have been associated with cancers, including breast and prostate cancer, highlighting its potential as a therapeutic target. Additionally, its genomic locus overlaps with regions prone to deletions or duplications in neurodevelopmental disorders, making recombinant ZNF277 a tool to study genotype-phenotype correlations. Current research focuses on mapping its target genes, signaling pathways, and post-translational modifications, such as ubiquitination, that modulate its activity. These efforts aim to clarify its contributions to disease mechanisms and normal cellular processes.
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