| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF283 |
| Uniprot No | Q8N7M2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679 aa |
| 活性数据 | MESRSVAQAG VQWCDLGSLQ APPPGFTLFS CLSLLSSWDY SSGFSGFCAS PIEESHGALI SSCNSRTMTD GLVTFRDVAI DFSQEEWECL DPAQRDLYVD VMLENYSNLV SLDLESKTYE TKKIFSENDI FEINFSQWEM KDKSKTLGLE ASIFRNNWKC KSIFEGLKGH QEGYFSQMII SYEKIPSYRK SKSLTPHQRI HNTEKSYVCK ECGKACSHGS KLVQHERTHT AEKHFECKEC GKNYLSAYQL NVHQRFHTGE KPYECKECGK TFSWGSSLVK HERIHTGEKP YECKECGKAF SRGYHLTQHQ KIHTGVKSYK CKECGKAFFW GSSLAKHEII HTGEKPYKCK ECGKAFSRGY QLTQHQKIHT GKKPYECKIC GKAFCWGYQL TRHQIFHTGE KPYECKECGK AFNCGSSLIQ HERIHTGEKP YECKECGKAF SRGYHLSQHQ KIHTGEKPFE CKECGKAFSW GSSLVKHERV HTGEKSHECK ECGKTFCSGY QLTRHQVFHT GEKPYECKEC GKAFNCGSSL VQHERIHTGE KPYECKECGK AFSRGYHLTQ HQKIHTGEKP FKCKECGKAF SWGSSLVKHE RVHTNEKSYE CKDCGKAFGS GYQLSVHQRF HTGEKLYQRK EFGKTFTCGS KLVHERTHSN DKPYKYNECG EAFLWTTYSN EKIDTDETL |
| 分子量 | 77.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF283蛋白的模拟参考文献示例(请注意，这些为假设性文献，实际检索时可能需要结合具体数据库验证)：
---
1. **文献名称**：*Cloning and Functional Characterization of Human ZNF283 as a Transcriptional Repressor*
**作者**：Smith, J. et al.
**摘要**：本研究成功克隆并重组表达了人ZNF283蛋白，发现其通过KRAB结构域介导转录抑制活性，可能在表观遗传调控中发挥作用。
2. **文献名称**：*Recombinant ZNF283 Protein Modulates Apoptosis in Cancer Cell Lines*
**作者**：Lee, H. & Chen, W.
**摘要**：通过原核系统表达重组ZNF283蛋白，发现其与Bcl-2家族蛋白相互作用，影响癌细胞凋亡通路，提示其在肿瘤中的潜在功能。
3. **文献名称**：*Structural Analysis of ZNF283 Zinc Finger Domain via Recombinant Protein Expression*
**作者**：Wang, Y. et al.
**摘要**：利用重组技术获得ZNF283锌指结构域蛋白，结合X射线晶体学解析其三维结构，揭示了其DNA结合特异性的分子机制。
4. **文献名称**：*ZNF283 Expression and Its Role in Neuronal Differentiation*
**作者**：Garcia, R. et al.
**摘要**：通过哺乳动物细胞系重组表达ZNF283.证明其通过调控靶基因(如NeuroD1)促进神经干细胞向神经元分化。
---
建议在实际研究中，通过PubMed、Google Scholar等平台，以“ZNF283 recombinant protein”“ZNF283 function”等关键词检索最新文献。若研究较少，可扩展至锌指蛋白家族(如ZNFs)或KRAB结构域蛋白相关研究。
Zinc finger protein 283 (ZNF283), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is encoded by the ZNF283 gene located on human chromosome 19q13.43. It belongs to the C2H2-type zinc finger transcription factor superfamily, characterized by tandem arrays of zinc finger motifs that bind DNA or RNA to regulate gene expression. ZNF283 is implicated in transcriptional regulation, chromatin remodeling, and cellular differentiation, though its precise biological functions remain understudied.
Recombinant human ZNF283 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling structural and functional studies. Its KRAB domain suggests potential involvement in repressing gene expression by recruiting chromatin-modifying complexes. Research highlights its interaction with retrotransposons and endogenous retroviruses, pointing to a role in genome stability and antiviral defense. Notably, ZNF283 binds to HIV-1 long terminal repeats (LTRs), hinting at regulatory effects on viral replication. Dysregulation of ZNF283 has been observed in cancers, though its oncogenic or tumor-suppressive mechanisms are unclear.
As a recombinant protein, ZNF283 serves as a tool for elucidating zinc finger protein-DNA interactions, epigenetic modulation, and disease-related pathways. Further studies are needed to clarify its physiological targets and therapeutic potential.
×
