| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF280C |
| Uniprot No | Q8ND82 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-737 aa |
| 活性数据 | MDDDKPFQPK NISKMAELFM ECEEEELEPW QKKVEETQDE DDDELIFVGE ISSSKPAISN ILNRGHSSSS SKGIKSEPHS PGIPEIFRTA SQRCRDPPSN PVAASPRFHL VSKSSQSSVT VENASKPDFT KNSQVGSDNS SILLFDSTQE SLPPSQDIPA IFREGMKNTS YVLKHPSTSK VNSVTPKKPK TSEDVPQINP STSLPLIGSP PVTSSQVMLS KGTNTSSPYD AGADYLRACP KCNVQFNLLD PLKYHMKHCC PDMITKFLGV IVKSERPCDE DKTDSETGKL IMLVNEFYYG RHEGVTEKEP KTYTTFKCFS CSKVLKNNIR FMNHMKHHLE LEKQNNESWE NHTTCQHCYR QYPTPFQLQC HIESTHTPHE FSTICKICEL SFETEHILLQ HMKDTHKPGE MPYVCQVCQF RSSTFSDVEA HFRAAHENTK NLLCPFCLKV SKMATPYMNH YMKHQKKGVH RCPKCRLQFL TSKEKAEHKA QHRTFIKPKE LEGLPPGAKV TIRASLGPLQ SKLPTAPFGC APGTSFLQVT PPTSQNTTAR NPRKSNASRS KTSKLHATTS TASKVNTSKP RGRIAKSKAK PSYKQKRQRN RKNKMSLALK NIRCRRGIHK CIECHSKIKD FASHFSIYIH CSFCKYNTNC NKAFVNHMMS SHSNHPGKRF CIFKKHSGTL RGITLVCLKC DFLADSSGLD RMAKHLSQRK THTCQVIIEN VSKSTSTSEP TTGCSLK |
| 分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人ZNF280C蛋白的参考文献示例(注：部分信息为示例性质，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Structural and functional characterization of recombinant human ZNF280C protein*
**作者**：Zhang L, et al. (2015)
**摘要**：本研究成功克隆并表达重组人ZNF280C蛋白，通过体外实验鉴定其C端锌指结构域在DNA结合中的关键作用，并验证其可能参与转录调控。
2. **文献名称**：*ZNF280C promotes colorectal cancer metastasis via PI3K/AKT signaling pathway*
**作者**：Chen H, et al. (2018)
**摘要**：通过重组ZNF280C蛋白过表达实验，发现其通过激活PI3K/AKT通路增强结直肠癌细胞侵袭和转移能力，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*Overexpression of ZNF280C correlates with poor prognosis in breast cancer*
**作者**：Wang Y, et al. (2021)
**摘要**：在乳腺癌组织中检测到ZNF280C高表达，利用重组蛋白体外实验证实其通过抑制凋亡相关基因促进肿瘤细胞存活，与患者不良预后显著相关。
4. **文献名称**：*Zinc finger proteins in genome editing: Insights from ZNF280C and CRISPR-Cas9 synergy*
**作者**：Lee S, et al. (2020)
**摘要**：综述锌指蛋白家族(含ZNF280C)的结构特征及重组表达技术，探讨其与CRISPR系统结合在基因编辑中的潜在应用。
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**注意**：以上文献为示例性描述，实际研究中可能需要根据具体研究方向检索PubMed、Web of Science或Google Scholar等数据库获取真实参考文献。建议结合关键词“recombinant ZNF280C”或“ZNF280C protein function”进一步筛选。
Zinc finger protein 280C (ZNF280C), a member of the zinc finger protein family, is encoded by a gene located on human chromosome 2p21. This protein belongs to the Cys2-His2 (C2H2) zinc finger superfamily, characterized by conserved zinc-binding motifs that enable sequence-specific DNA or RNA interactions. ZNF280C shares structural homology with ZNF280B, forming heterodimers that may influence transcriptional regulation. Initially identified through genomic sequencing, its expression is detected in various tissues, with elevated levels observed in testis and certain cancers, suggesting potential roles in reproduction and tumorigenesis.
Emerging studies link ZNF280C to cancer progression. CRISPR-Cas9 screens revealed its functional importance in prostate cancer cell survival, while overexpression in colorectal cancer correlates with advanced tumor stages and poor prognosis. Mechanistically, ZNF280C is proposed to regulate cell proliferation and metastasis through pathways like PI3K/AKT signaling. Nuclear localization signals in its structure align with potential transcriptional regulatory functions. Structural analysis predicts homodimerization capacity and DNA-binding specificity, though exact target genes remain under investigation.
Recombinant ZNF280C protein enables biochemical studies to decode its interactome, post-translational modifications, and DNA-binding properties. Current research focuses on validating its oncogenic potential and exploring therapeutic targeting strategies in malignancies. The protein’s dual nuclear/cytoplasmic distribution adds complexity to understanding its context-dependent roles in cellular homeostasis and disease.
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