| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF267 |
| Uniprot No | Q14586 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743 aa |
| 活性数据 | MGLLTFRDVA VEFSLEEWEH LEPAQKNLYQ DVMLENYRNL VSLGLVVSKP DLITFLEQRK EPWNVKSEET VAIQPDVFSH YNKDLLTEHC TEASFQKVIS RRHGSCDLEN LHLRKRWKRE ECEGHNGCYD EKTFKYDQFD ESSVESLFHQ QILSSCAKSY NFDQYRKVFT HSSLLNQQEE IDIWGKHHIY DKTSVLFRQV STLNSYRNVF IGEKNYHCNN SEKTLNQSSS PKNHQENYFL EKQYKCKEFE EVFLQSMHGQ EKQEQSYKCN KCVEVCTQSL KHIQHQTIHI RENSYSYNKY DKDLSQSSNL RKQIIHNEEK PYKCEKCGDS LNHSLHLTQH QIIPTEEKPC KWKECGKVFN LNCSLYLTKQ QQIDTGENLY KCKACSKSFT RSSNLIVHQR IHTGEKPYKC KECGKAFRCS SYLTKHKRIH TGEKPYKCKE CGKAFNRSSC LTQHQTTHTG EKLYKCKVCS KSYARSSNLI MHQRVHTGEK PYKCKECGKV FSRSSCLTQH RKIHTGENLY KCKVCAKPFT CFSNLIVHER IHTGEKPYKC KECGKAFPYS SHLIRHHRIH TGEKPYKCKA CSKSFSDSSG LTVHRRTHTG EKPYTCKECG KAFSYSSDVI QHRRIHTGQR PYKCEECGKA FNYRSYLTTH QRSHTGERPY KCEECGKAFN SRSYLTTHRR RHTGERPYKC DECGKAFSYR SYLTTHRRSH SGERPYKCEE CGKAFNSRSY LIAHQRSHTR EKL |
| 分子量 | 87.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人ZNF267蛋白的参考文献示例(注：文献为模拟生成，非真实存在)：
1. **《ZNF267基因的克隆及其在肿瘤细胞中的表达调控》**
作者：Zhang L, et al.
摘要：研究报道了人源ZNF267蛋白的重组表达及纯化方法，发现其在胃癌组织中异常高表达，可能通过调控Wnt信号通路促进肿瘤侵袭。
2. **《Structural characterization of ZNF267 zinc finger domain and DNA binding specificity》**
作者：Chen W, et al.
摘要：通过X射线晶体学解析了ZNF267蛋白锌指结构域的三维结构，揭示了其特异性结合DNA基序的分子机制。
3. **《ZNF267 inhibits neuronal apoptosis via epigenetic regulation of pro-survival genes》**
作者：Kimura T, et al.
摘要：研究利用重组ZNF267蛋白证明其通过招募组蛋白修饰酶HDAC1调控靶基因转录，在阿尔茨海默病模型中抑制神经元凋亡。
4. **《Recombinant ZNF267 promotes cancer stemness in hepatocellular carcinoma》**
作者：Wang Y, et al.
摘要：实验证实重组ZNF267蛋白可通过激活STAT3信号通路增强肝癌干细胞自我更新能力，提示其作为治疗靶点的潜力。
Zinc finger protein 267 (ZNF267) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem Cys₂-His₂ (C2H2) zinc finger motifs that facilitate sequence-specific DNA binding. As a transcription factor, ZNF267 is implicated in regulating gene expression by interacting with promoter regions or epigenetic modifiers. It is ubiquitously expressed in human tissues, with roles in cellular processes such as differentiation, proliferation, and apoptosis. Structurally, the protein comprises an N-terminal KRAB domain, known to recruit transcriptional co-repressors like KAP1. and multiple C-terminal zinc finger repeats determining DNA-binding specificity.
Research links ZNF267 to cancer progression, particularly in gastrointestinal malignancies. Overexpression has been observed in gastric and colorectal cancers, where it potentially activates oncogenic pathways or represses tumor suppressors. Its dysregulation may also contribute to chemoresistance. Recombinant ZNF267 protein, typically produced in *E. coli* or mammalian systems with tags (e.g., GST, His-tag), enables *in vitro* studies on DNA-protein interactions, structural analysis, and inhibitor screening. Current investigations focus on elucidating its target genes, regulatory networks, and therapeutic potential. However, mechanistic insights into its precise biological functions and disease associations remain incomplete, warranting further exploration.
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