| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF274 |
| Uniprot No | Q96GC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653 aa |
| 活性数据 | MASRLPTAWS CEPVTFEDVT LGFTPEEWGL LDLKQKSLYR EVMLENYRNL VSVEHQLSKP DVVSQLEEAE DFWPVERGIP QDTIPEYPEL QLDPKLDPLP AESPLMNIEV VEVLTLNQEV AGPRNAQIQA LYAEDGSLSA DAPSEQVQQQ GKHPGDPEAA RQRFRQFRYK DMTGPREALD QLRELCHQWL QPKARSKEQI LELLVLEQFL GALPVKLRTW VESQHPENCQ EVVALVEGVT WMSEEEVLPA GQPAEGTTCC LEVTAQQEEK QEDAAICPVT VLPEEPVTFQ DVAVDFSREE WGLLGPTQRT EYRDVMLETF GHLVSVGWET TLENKELAPN SDIPEEEPAP SLKVQESSRD CALSSTLEDT LQGGVQEVQD TVLKQMESAQ EKDLPQKKHF DNRESQANSG ALDTNQVSLQ KIDNPESQAN SGALDTNQVL LHKIPPRKRL RKRDSQVKSM KHNSRVKIHQ KSCERQKAKE GNGCRKTFSR STKQITFIRI HKGSQVCRCS ECGKIFRNPR YFSVHKKIHT GERPYVCQDC GKGFVQSSSL TQHQRVHSGE RPFECQECGR TFNDRSAISQ HLRTHTGAKP YKCQDCGKAF RQSSHLIRHQ RTHTGERPYA CNKCGKAFTQ SSHLIGHQRT HNRTKRKKKQ PTS |
| 分子量 | 74.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF274蛋白的3篇参考文献的简要整理：
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1. **文献名称**：*ZNF274 interacts with TRIM28 to silence endogenous retrovirones in human cells*
**作者**：Trono D. et al.
**摘要**：该研究揭示了ZNF274通过与TRIM28蛋白形成复合物，招募表观遗传修饰酶(如SETDB1)，靶向抑制人类基因组中的内源性逆转录病毒元件，维持基因组稳定性。
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2. **文献名称**：*Biochemical characterization of the zinc finger protein ZNF274 and its role in chromatin interaction*
**作者**：Farnham P.J. et al.
**摘要**：研究利用重组ZNF274蛋白进行体外实验，证实其通过锌指结构域特异性结合异染色质区域(如端粒附近的重复序列)，并可能通过染色质重塑复合物参与基因沉默。
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3. **文献名称**：*The KRAB-Zinc Finger Protein ZNF274 Recruits the Histone Methyltransferase SETDB1 to Regulate Gene Expression*
**作者**：Yang L. et al.
**摘要**：本文通过重组蛋白互作实验，证明ZNF274的KRAB结构域直接结合SETDB1甲基转移酶，介导H3K9me3表观标记，调控发育相关基因的表达模式。
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**注**：以上文献信息为模拟示例，实际引用建议通过PubMed或Web of Science核实。研究重点包括ZNF274在表观遗传调控、基因沉默及染色质相互作用中的分子机制。
ZNF274. a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is a transcription factor involved in transcriptional repression and heterochromatin formation. Characterized by multiple C2H2-type zinc finger motifs, it binds to specific DNA sequences, particularly in gene-poor regions like pericentromeric heterochromatin and telomeres. The protein interacts with the co-repressor TRIM28 (KAP1) through its KRAB domain, recruiting histone methyltransferase SETDB1 to catalyze H3K9 trimethylation, thereby promoting chromatin condensation and gene silencing. This mechanism is critical for maintaining genomic stability and regulating repetitive element activity.
Recombinant human ZNF274 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It enables *in vitro* analyses of DNA-protein interactions, chromatin remodeling, and epigenetic regulation. Researchers utilize it to investigate its role in diseases linked to genomic instability, such as cancers and aging-related disorders. Its applications span ChIP-seq, electrophoretic mobility shift assays (EMSAs), and CRISPR-based transcriptional repression studies. The recombinant form retains DNA-binding specificity and functional domains, making it a valuable tool for dissecting zinc finger protein mechanisms and developing targeted epigenetic therapies.
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