| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF284 |
| Uniprot No | Q2VY69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593 aa |
| 活性数据 | MTMFKEAVTF KDVAVVFTEE ELGLLDVSQR KLYRDVMLEN FRNLLSVGHQ LSHRDTFHFQ REEKFWIMET ATQREGNSGG KIQTELESVP ETGPHEEWSC QQIWEQTASE LTRPQDSISS SQFSTQGDVP SQVDAGLSII HIGETPSEHG KCKKFFSDVS ILDLHQQLHS GKISHTCNEY RKRFCYSSAL CLHQKVHMGE KRYKCDVCSK AFSQNSQLQT HQRIHTGEKP FKCEQCGKSF SRRSGMYVHC KLHTGEKPHI CEECGKAFIH NSQLREHQRI HTGEKPFKCY ICGKSFHSRS NLNRHSMVHM QEKSFRCDTC SNSFGQRSAL NSHCMDHTKE KLYKCEECGR SFTCRQDLCK HQMDHTGDKP YNCNVCGKGF RWSSCLSRHQ RVHNGETTFK CDGCGKRFYM NSQGHSHQRA YREEELYKCQ KCGKGYISKF NLDLHQRVHT GERPYNCKEC GKSFRWASGI LRHKRLHTGE KPFKCEECGK RFTENSKLRF HQRIHTGEKP YKCEECGKGF RWASTHLTHQ RLHSREKLFQ CEDCGKSSEH SSCLQDQQSD HSGEKTSKCE DCGKRYERRL NLDMILSLFL NDI |
| 分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF284蛋白的假设性参考文献(仅供示例参考)：
1. **文献名称**: "Cloning and functional characterization of recombinant human ZNF284 protein"
**作者**: Smith J, et al.
**摘要**: 本研究成功克隆并表达了重组人ZNF284蛋白，通过体外实验证明其具有特异性DNA结合能力，可能在基因转录调控中发挥作用。
2. **文献名称**: "ZNF284 interacts with leukemia-associated chromosomal translocation proteins"
**作者**: Zhang Y, et al.
**摘要**: 报道重组ZNF284蛋白与AML1-ETO融合蛋白的相互作用，提示其在急性髓系白血病发生中的潜在调控机制。
3. **文献名称**: "Structural analysis of the zinc finger domains in ZNF284"
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析重组ZNF284蛋白的锌指结构域，揭示其与靶DNA序列结合的分子基础。
4. **文献名称**: "ZNF284 overexpression suppresses colorectal cancer cell proliferation"
**作者**: Chen L, et al.
**摘要**: 实验利用重组ZNF284蛋白验证其在结直肠癌细胞中通过抑制Wnt/β-catenin信号通路降低肿瘤活性。
(注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。)
Zinc finger protein 284 (ZNF284) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that facilitate sequence-specific DNA binding. As a transcription factor, ZNF284 is hypothesized to regulate gene expression by interacting with promoter regions of target genes, though its precise biological roles remain incompletely understood. It is encoded in humans by the *ZNF284* gene located on chromosome 19 (19q13.43) and is thought to participate in cellular processes such as proliferation, differentiation, or apoptosis, albeit with limited mechanistic evidence.
Recombinant human ZNF284 protein is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its structural features, including tandem zinc finger motifs, make it valuable for investigating DNA-protein interactions and transcriptional regulation networks. Research has explored its potential involvement in disease contexts, such as cancer, where dysregulated zinc finger proteins often contribute to oncogenesis or tumor suppression. However, ZNF284’s specific targets, binding partners, and regulatory pathways remain understudied compared to other zinc finger proteins.
Current applications of recombinant ZNF284 include in vitro DNA-binding assays, chromatin immunoprecipitation (ChIP) studies, and screening for epigenetic modifiers. Efforts to map its binding sites genome-wide or elucidate post-translational modifications may clarify its role in health and disease, offering avenues for therapeutic targeting in conditions linked to transcriptional dysregulation.
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