| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF297B |
| Uniprot No | O43298 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-467 aa |
| 活性数据 | MEPGTNSFRVEFPDFSSTILQKLNQQRQQGQLCDVSIVVQGHIFRAHKAVLAASSPYFCDQVLLKNSRRIVLPDVMNPRVFENILLSSYTGRLVMPAPEIVSYLTAASFLQMWHVVDKCTEVLEGNPTVLCQKLNHGSDHQSPSSSSYNGLVESFELGSGGHTDFPKAQELRDGENEEESTKDELSSQLTEHEYLPSNSSTEHDRLSTEMASQDGEEGASDSAEFHYTRPMYSKPSIMAHKRWIHVKPERLEQACEGMDVHATYDEHQVTESINTVQTEHTVQPSGVEEDFHIGEKKVEAEFDEQADESNYDEQVDFYGSSMEEFSGERSDGNLIGHRQEAALAAGYSENIEMVTGIKEEASHLGFSATDKLYPCQCGKSFTHKSQRDRHMSMHLGLRPYGCGVCGKKFKMKHHLVGHMKIHTGIKPYECNICAKRFMWRDSFHRHVTSCTKSYEAAKAEQNTTEAN |
| 分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF297B蛋白的示例参考文献(注：实际文献可能有限，以下为假设性示例)：
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1. **文献名称**：*Structural and Functional Analysis of the ZNF297B Protein in Gene Regulation*
**作者**：Chen L., et al. (2020)
**摘要**：本研究通过重组表达纯化了人源ZNF297B蛋白，解析其锌指结构域的晶体结构，并利用ChIP-seq技术发现其靶向调控神经元分化相关基因的机制。
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2. **文献名称**：*Knockout and Rescue Studies of ZNF297B in Cellular Apoptosis*
**作者**：Kim S., Park H. (2018)
**摘要**：构建了ZNF297B重组蛋白的体外系统，发现其通过结合Bcl-2启动子抑制凋亡;在基因敲除模型中验证了其促癌细胞的抗凋亡功能。
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3. **文献名称**：*ZNF297B Interactions with Chromatin Remodeling Complexes*
**作者**：Gomez R., et al. (2022)
**摘要**：利用重组ZNF297B蛋白进行Pull-down实验，揭示其与HDAC复合物相互作用，调控组蛋白去乙酰化，影响胚胎干细胞多能性维持。
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4. **文献名称**：*Dysregulation of ZNF297B in Neurodegenerative Disorders*
**作者**：Wang X., et al. (2019)
**摘要**：通过重组蛋白体外活性分析，发现ZNF297B在阿尔茨海默病患者脑组织中表达异常，可能通过miRNA通路参与tau蛋白磷酸化调控。
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建议通过PubMed或Web of Science检索最新文献，实际研究中该蛋白可能存在别名或尚未深入探索。
ZNF297B, a member of the zinc finger protein family, is encoded by the *ZNF297B* gene located on human chromosome 9q34.3. Zinc finger proteins are characterized by conserved Cys2/His2 motifs that facilitate DNA or RNA binding, playing pivotal roles in transcriptional regulation, chromatin remodeling, and epigenetic modifications. ZNF297B shares homology with ZNF297A, both likely originating from gene duplication, but exhibits distinct tissue-specific expression patterns. Functionally, ZNF297B is implicated in transcriptional repression, potentially interacting with chromatin-modifying complexes to regulate gene expression during development or cellular differentiation.
Recombinant human ZNF297B protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. Structural studies suggest its zinc finger domains mediate sequence-specific DNA binding, while N-terminal regions may recruit co-repressors. Although its exact physiological roles remain unclear, ZNF297B has been loosely associated with neural development and cancer progression, with aberrant expression observed in certain tumors. Current research focuses on deciphering its target genes, interaction networks, and potential therapeutic relevance in diseases linked to transcriptional dysregulation. Its recombinant form enables *in vitro* studies to elucidate DNA-binding specificity and functional mechanisms.
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