| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF319 |
| Uniprot No | Q9P2F9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582 aa |
| 活性数据 | MSESWQQPPQ TQPQQPQPPQ PQHHAEPPPA LAEHTLPPGT AENPLGCAVY GILLQPDPGL QPPQHAPLQA AGEPGPKCGV CGHDLAHLSS PHEHQCLAGH DRSFQCTQCL KIFHQATDLL EHQCVQAEQK PFVCGVCKMG FSLLTSLAQH HSSHSGLVKC SICEKTYKPA EAAEPATTAA PSLPAAPAPS TVTPAEQADK PYSCPICQKP FKHLSELSRH ERIHTGEKPY KCTLCDKSFS QSSHLVHHKR THSSERPYKC AVCEKTFKHR SHLVRHMYAH SGEHHLFRCN VCELHFKESS ELLQHPCTPS GERPFRCGEC QKAFKRPSDL RQHERTHSAE RPFKCDLCPM GFKQQYALMR HRRTHKTEEP FKCGLCEKGF GQPSHLLYHQ HVHTLETLFK CPVCQKGFDQ SAELLRHKCL PGAAERPFKC PVCNKAYKRA SALQKHQLAH CAAAEKPLRC TLCERRFFSS SEFVQHRCDP AREKPLKCPD CEKRFKYASD LQRHRRVHTG EKPYKCPNCD KAFKQREHLN KHQGVHAREQ QFKCVWCGER FLDVALLQEH SAQHSAAAAA AEGAYQVAAC LP |
| 分子量 | 65.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF319蛋白的虚构参考文献示例(实际文献可能需要通过学术数据库核实)：
1. **文献名称**：**《重组人ZNF319蛋白在DNA损伤应答中的功能研究》**
**作者**：Zhang L, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人ZNF319蛋白，并通过Pull-down实验证实其与DNA修复蛋白Ku70相互作用，提示ZNF319可能参与调控DNA损伤修复通路。
2. **文献名称**：**《ZNF319重组蛋白的晶体结构及其锌指结构域分析》**
**作者**：Wang Y, et al.
**摘要**：利用X射线衍射解析了ZNF319蛋白的晶体结构，揭示其C端含典型的锌指结构域，可能通过该结构结合特定DNA序列，影响靶基因转录调控。
3. **文献名称**：**《重组ZNF319抑制肺癌细胞增殖的机制探究》**
**作者**：Chen H, et al.
**摘要**：体外实验表明，重组表达的ZNF319蛋白可通过下调cyclin D1表达，阻滞肺癌细胞G1/S期进程，并诱导凋亡，提示其潜在的肿瘤抑制作用。
4. **文献名称**：**《重组ZNF319蛋白在慢性炎症中的免疫调节作用》**
**作者**：Kim S, et al.
**摘要**：通过哺乳动物细胞表达系统获得高活性ZNF319重组蛋白，发现其能够抑制NF-κB信号通路，减少促炎细胞因子分泌，可能成为炎症性疾病治疗靶点。
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**注**：以上内容为模拟示例，实际研究中ZNF319的文献可能较少。建议通过**PubMed/Google Scholar**以“ZNF319 recombinant”或“ZNF319 protein function”为关键词检索最新文献，或关注相关锌指蛋白家族的综述以获取间接参考。
Zinc finger protein 319 (ZNF319) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA or RNA interactions. First identified through genomic analysis, ZNF319 is encoded in humans on chromosome 16q22.1 and is evolutionarily conserved, suggesting functional importance. While its precise biological role remains under investigation, ZNF319 is hypothesized to act as a transcriptional regulator, potentially repressing gene expression via its KRAB domain, which recruits chromatin-modifying complexes. Emerging studies link ZNF319 to cellular processes such as cell cycle regulation, apoptosis, and differentiation, with altered expression observed in cancers (e.g., hepatocellular carcinoma, gastric cancer) and neurological disorders. Recombinant human ZNF319 protein, produced in heterologous systems like *E. coli* or mammalian cells, enables *in vitro* studies of its DNA-binding specificity, protein interactome, and regulatory mechanisms. Its recombinant form is crucial for structural analyses (e.g., X-ray crystallography) and functional assays to map domain-specific activities. Though therapeutic applications remain exploratory, ZNF319's involvement in disease pathways positions it as a potential biomarker or drug target. Current research focuses on elucidating its target genes, signaling networks (e.g., TGF-β, Wnt), and context-dependent roles in development and pathology. Limited clinical data and incomplete mechanistic understanding highlight the need for further investigation.
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