| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF306 |
| Uniprot No | Q9BRR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538 aa |
| 活性数据 | MARELSESTALDAQSTEDQMELLVIKVEEEEAGFPSSPDLGSEGSRERFRGFRYPEAAGPREALSRLRELCRQWLQPEMHSKEQILELLVLEQFLTILPGNLQSWVREQHPESGEEVVVLLEYLERQLDEPAPQVSGVDQGQELLCCKMALLTPAPGSQSSQFQLMKALLKHESVGSQPLQDRVLQVPMLAHGGCCREDAVVASRLTPESQGLLKVEDVALTLTPEWTQQDSSQGNLCRDEKQENHGSLVSLGDEKQTKSRDLPPAEELPEKEHGKISCHLREDIAQIPTCAEAGEQEGRLQRKQKNATGGRRHICHECGKSFAQSSGLSKHRRIHTGEKPYECEECGKAFIGSSALVIHQRVHTGEKPYECEECGKAFSHSSDLIKHQRTHTGEKPYECDDCGRTFSQSCSLLEHHRIHTGEKPYQCSMCGKAFRRSSHLLRHQRIHTGDKNVQEPEQGEAWKSRMESQLENVETPMSYKCNECERSFTQNTGLIEHQKIHTGEKPYQCNACGKGFTRISYLVQHQRSHVGKNILSQ |
| 分子量 | 84.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF306蛋白的3篇假设性参考文献，基于典型研究方向的合理推测，供参考：
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1. **标题**：Structural Insights into the DNA-Binding Mechanism of Recombinant Human ZNF306
**作者**：Smith AE, et al.
**摘要**：本研究解析了重组人ZNF306蛋白的晶体结构，揭示了其C2H2型锌指结构域如何特异性识别DNA序列。通过体外结合实验，证实了ZNF306在调控靶基因转录中的潜在作用，为理解其生物学功能提供结构基础。
2. **标题**：ZNF306 Promotes Tumor Metastasis via Wnt/β-Catenin Signaling Pathway
**作者**：Zhang L, et al.
**摘要**：文章报道重组人ZNF306蛋白在结直肠癌细胞中过表达，通过激活Wnt/β-catenin信号通路增强肿瘤侵袭性。体外实验表明，ZNF308可通过重组蛋白直接与β-catenin相互作用，提示其作为癌症治疗靶标的可能性。
3. **标题**：Functional Characterization of Recombinant ZNF306 in Epigenetic Silencing
**作者**：Chen X, et al.
**摘要**：利用重组ZNF306蛋白进行染色质免疫沉淀测序(ChIP-seq)，发现其与Polycomb抑制复合物(PRC2)协同调控基因沉默，尤其在干细胞分化中起关键作用。研究揭示了ZNF306在表观遗传调控中的新机制。
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**备注**：以上文献为假设性示例，实际研究中建议通过PubMed、Google Scholar等平台以“ZNF306”“recombinant”等关键词检索最新成果。如需真实文献，可进一步提供检索结果。
Zinc finger protein 306 (ZNF306), also known as JAZ or ZFP306. is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by tandem C₂H₂-type zinc finger motifs and an N-terminal KRAB domain. This nuclear protein is evolutionarily conserved across vertebrates and plays regulatory roles in transcriptional repression through its KRAB domain, which mediates protein-protein interactions, and zinc finger motifs that bind specific DNA sequences. Studies suggest its involvement in diverse biological processes, including cell differentiation, proliferation, and apoptosis, with potential links to embryonic development and oncogenesis. Mouse models show ZNF306 expression in multiple tissues, particularly during embryogenesis.
As a recombinant protein, ZNF306 is typically produced in Escherichia coli or mammalian expression systems for functional studies. Its recombinant form enables research into DNA-binding specificity, interaction partners, and regulatory pathways. Emerging evidence implicates ZNF306 in tumor progression through mechanisms like epithelial-mesenchymal transition modulation, though its exact pathophysiological roles remain under investigation. In biomedical research, recombinant ZNF306 serves as a tool for elucidating zinc finger protein functions, developing cellular models, and exploring therapeutic targets, particularly in cancer biology and developmental disorders.
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