| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF31 |
| Uniprot No | P17040 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-433 aa |
| 活性数据 | MAMALELQAQASPQPEPEELLIVKLEEDSWGSESKLWEKDRGSVSGPEASRQRFRQFQYRDAAGPHEAFSQLWALCCRWLRPEIRLKEQILELLVLEQFLTILPREVQTWVQARHPESGEEAVALVEDWHRETRTAGQSGLELHTEETRPLKTGEEAQSFQLQPVDPWPEGQSQKKGVKNTCPDLPNHLNAEVAPQPLKESGVPVSKPSNTSEKEQGPEFWGLSLINSGKRSTADYSLDNEPAQALTWRDSRAWEEQYQWDVEDMKVSGVHWGYEETKTFLAILSESPFSEKLRTCHQNRQVYRAIAEQLRARGFLRTLEQCRYRVKNLLRNYRKAKSSHPPGTCPFYEELEALVRARTAIRATDGPGEAVALPRLGYSDAEMDEQEEGGWDPEEMAEDCNGAGLVNVESTQGPRIAGAPALFQSRIGKNMGV |
| 分子量 | 117.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF31蛋白的3篇参考文献，基于真实研究领域常见的主题和作者推断(若需要具体文献，建议通过学术数据库进一步筛选)：
1. **文献名称**：*"ZNF31 regulates the proliferation and invasion of breast cancer cells through the PI3K/AKT signaling pathway"*
**作者**：Wang L, et al.
**摘要**：研究探讨了ZNF31在乳腺癌细胞中的功能，通过重组蛋白实验发现ZNF31过表达激活PI3K/AKT通路，促进细胞增殖和侵袭。
2. **文献名称**：*"Structural characterization and DNA-binding properties of the human zinc finger protein ZNF31"*
**作者**：Kim H, et al.
**摘要**：利用重组ZNF31蛋白解析其锌指结构域的三维结构，揭示其特异性结合DNA的分子机制，并验证其在基因转录调控中的作用。
3. **文献名称**：*"Recombinant expression and functional analysis of ZNF31 in the regulation of immune response"*
**作者**：Zhang Y, et al.
**摘要**：成功在大肠杆菌中表达重组ZNF31蛋白，实验表明其可通过调控炎症因子表达(如TNF-α、IL-6)参与巨噬细胞的免疫应答。
4. **文献名称**：*"ZNF31 interacts with HDAC1 to suppress epithelial-mesenchymal transition in gastric cancer"*
**作者**：Liu X, et al.
**摘要**：通过重组蛋白共沉淀实验证实ZNF31与HDAC1的相互作用，并发现其通过抑制EMT过程降低胃癌转移能力。
**注意**：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science等平台以“ZNF31”或“Zinc Finger Protein 31”为关键词检索获取。部分研究可能未直接使用重组蛋白，但可通过原文方法学进一步确认。
**Background of Recombinant Human ZNF31 Protein**
Zinc finger protein 31 (ZNF31), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZFP) family, is encoded by the *ZNF31* gene in humans. ZNF31 functions as a transcriptional regulator, characterized by its C2H2-type zinc finger motifs, which enable sequence-specific DNA binding. These motifs, typically arranged in tandem repeats, mediate interactions with target gene promoters or regulatory elements, often repressing transcription through recruitment of co-repressor complexes. The KRAB domain at the N-terminal region further facilitates interactions with chromatin-modifying enzymes, contributing to epigenetic regulation.
ZNF31 is implicated in diverse cellular processes, including cell proliferation, differentiation, and apoptosis. While its precise physiological roles remain under investigation, studies suggest involvement in immune response modulation and cancer progression. For instance, dysregulation of ZNF31 has been observed in certain malignancies, linking it to oncogenic or tumor-suppressive pathways depending on cellular context.
Recombinant ZNF31 protein is typically produced using *E. coli* or mammalian expression systems, followed by purification via affinity chromatography. This engineered protein serves as a vital tool for elucidating ZNF31's molecular mechanisms, DNA-binding specificity, and interactions with partner proteins. Applications extend to *in vitro* assays (e.g., electrophoretic mobility shift assays), structural studies, and screening for potential therapeutic compounds targeting ZNF31-associated pathways. Ongoing research aims to clarify its role in disease and exploit its regulatory functions for biomedical interventions.
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