纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | ZNF354B |
Uniprot No | Q96LW1 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-612 aa |
活性数据 | MAAGQREARP QVSLTFEDVA VLFTWDEWRK LAPSQRNLYR DVMLENYRNL VSLGLSFTKP KVISLLQQGE DPWEVEKDSS GVSSLGCKST PKMTKSTQTQ DSFQEQIRKR LKRDEPWNFI SERSCIYEEK LKKQQDKNEN LQIISVAHTK ILTVDRSHKN VEFGQNFYLK SVFIKQQRFA KEKTPSKCEI QRNSFKQNSN LLNQSKIKTA EKRYKCSTCE KAFIHNSSLR KHQKNHTGEK LFKCKECLKA FSQSSALIQH QRTHTGEKPY ICKECGKAFS HSASLCKHLR THTVEKCYRC KECGKSFSRR SGLFIHQKIH AQENPHKYNP GRKASSYSTS LSGSQKIHLR KKSYLCNECG NTFKSSSSLR YHQRIHTGEK PFKCSECGRA FSQSASLIQH ERIHTGEKPY RCNECGKGFT SISRLNRHRI IHTGEKLYNC NECGKALSSH STLIIHERIH TGEKPCKCKV CGKAFRQSSA LIQHQRMHTG ERPYKCNECD KTFRCNSSLS NHQRIHTGEK PYRCLECGMS FGQSAALIQH QRIHTGEKPF KCNTCGKTFR QSSSLIAHQR IHTGEKPYEC NACGKLFSQR SSLTNHYKIH IEEDSLKADL HV |
分子量 | 70.5 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性参考案例(实际文献需通过学术数据库验证):
1. **文献名称**:*ZNF354B functions as a transcriptional repressor in neuronal differentiation*
**作者**:Smith J, et al.
**摘要**:研究利用重组人ZNF354B蛋白发现其通过结合特定DNA序列抑制神经分化相关基因,其KRAB结构域参与招募组蛋白去乙酰化酶复合体。
2. **文献名称**:*Structural analysis of recombinant ZNF354B reveals zinc finger-mediated DNA binding specificity*
**作者**:Chen L, et al.
**摘要**:通过体外表达纯化ZNF354B蛋白,结合晶体学分析揭示其锌指结构识别DNA基序的分子机制,并验证其与靶基因启动子区域的结合能力。
3. **文献名称**:*Dysregulation of ZNF354B in colorectal cancer links epigenetic silencing to tumor progression*
**作者**:Wang Y, et al.
**摘要**:研究发现结直肠癌中ZNF354B表达下调,体外实验表明重组ZNF354B过表达可抑制肿瘤细胞增殖,机制涉及甲基化依赖的抑癌基因激活。
4. **文献名称**:*ZNF354B interacts with HDAC1/2 to regulate oxidative stress response*
**作者**:Kim T, et al.
**摘要**:通过免疫共沉淀及重组蛋白实验证实ZNF354B与HDAC1/2形成复合物,调控抗氧化应激相关基因(如SOD2)的表达,影响细胞存活。
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注:以上内容为基于锌指蛋白家族典型研究的模拟案例,实际文献需通过PubMed、Web of Science等平台检索确认。
Zinc finger protein 354B (ZNF354B), also known as testis zinc finger protein, is a Krüppel-associated box (KRAB)-containing zinc finger protein predominantly expressed in the testis and certain cancer tissues. As a member of the zinc finger protein family, it features multiple C2H2-type zinc finger domains that enable sequence-specific DNA binding, suggesting a role in transcriptional regulation. The KRAB domain, located at the N-terminus, typically mediates interactions with co-repressors to recruit chromatin-modifying complexes, thereby repressing gene expression.
Studies indicate ZNF354B may function as a transcriptional repressor involved in cell differentiation, apoptosis, and tumorigenesis. Its restricted expression pattern in germ cells implies potential roles in spermatogenesis or germ cell development. Aberrant expression has been observed in cancers, with evidence suggesting tumor-suppressive properties in prostate cancer through epigenetic silencing of oncogenic pathways. However, its precise biological mechanisms remain poorly characterized.
Recombinant ZNF354B protein is often produced in Escherichia coli or mammalian expression systems for in vitro studies, enabling investigations into DNA-binding specificity, protein-protein interactions, and functional assays. Ongoing research aims to elucidate its regulatory targets, physiological relevance in reproduction, and therapeutic implications in cancer biology. Limited structural and functional data highlight the need for further exploration of this understudied protein.
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