| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF345 |
| Uniprot No | Q14585 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-488 aa |
| 活性数据 | MENLTKHSIE CSSFRGDWEC KNQFERKQGS QEGHFSEMIF TPEDMPTFSI QHQRIHTDEK LLECKECGKD FSFVSVLVRH QRIHTGEKPY ECKECGKAFG SGANLAYHQR IHTGEKPFEC KECGKAFGSG SNLTHHQRIH TGEKPYECKE CGKAFSFGSG LIRHQIIHSG EKPYECKECG KSFSFESALI RHHRIHTGEK PYECIDCGKA FGSGSNLTQH RRIHTGEKPY ECKACGMAFS SGSALTRHQR IHTGEKPYIC NECGKAFSFG SALTRHQRIH TGEKPYVCKE CGKAFNSGSD LTQHQRIHTG EKPYECKECE KAFRSGSKLI QHQRMHTGEK PYECKECGKT FSSGSDLTQH HRIHTGEKPY ECKECGKAFG SGSKLIQHQL IHTGERPYEC KECGKSFSSG SALNRHQRIH TGEKPYECKE CGKAFYSGSS LTQHQRIHTG EKLYECKNCG KAYGRDSEFQ QHKKSHNGKK LCELETIN |
| 分子量 | 55.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF345蛋白的参考文献示例(注：由于ZNF345研究较少，部分内容可能基于领域内相关研究推测，建议进一步核实具体文献)：
1. **《ZNF345蛋白的克隆表达及DNA结合特性研究》**
- 作者：Smith J, et al.
- 摘要：本文首次报道了人源ZNF345基因的重组表达及纯化，通过大肠杆菌系统成功获得可溶性蛋白，并验证其特异性结合DNA保守序列的能力，提示其在转录调控中的潜在作用。
2. **《锌指蛋白ZNF345在乳腺癌细胞中的抑癌功能分析》**
- 作者：Zhang Y, et al.
- 摘要：利用重组ZNF345蛋白体外处理乳腺癌细胞，发现其显著抑制细胞增殖并诱导凋亡，可能通过调控p53通路发挥作用，为癌症治疗提供新靶点。
3. **《ZNF345蛋白结构解析及功能域研究》**
- 作者：Johnson R, et al.
- 摘要：通过X射线晶体学解析了重组ZNF350蛋白(作者注：此处可能是笔误，假设为ZNF345)的C端锌指结构域三维结构，揭示了其与DNA相互作用的分子机制。
4. **《人类锌指蛋白家族的功能多样性：从ZNF345到疾病关联》**
- 作者：Lee S, et al.
- 摘要：综述性文章提及ZNF345在基因编辑和表观遗传调控中的角色，并指出重组ZNF345蛋白可用于高通量筛选其相互作用分子。
**提示**：以上为模拟示例，ZNF345的实际研究可能较有限，建议通过PubMed或Google Scholar以“ZNF345 recombinant protein”“ZNF345 function”为关键词检索最新文献。
ZNF345. a member of the zinc finger protein family, is characterized by its conserved Cys2-His2 (C2H2) zinc finger domains, which facilitate sequence-specific DNA binding and nucleic acid interactions. This nuclear protein is postulated to function as a transcription regulator, potentially influencing gene expression through chromatin remodeling or direct promoter targeting. The zinc finger motifs, composed of tandem repeats, enable ZNF345 to recognize distinct DNA sequences, while auxiliary domains (e.g., KRAB or SCAN domains) may mediate protein-protein interactions or epigenetic complex recruitment. Recombinant ZNF345 is typically engineered using bacterial or mammalian expression systems with affinity tags (e.g., His-tag) for purification. Research focuses on elucidating its roles in cellular processes such as differentiation, apoptosis, and stress response. Notably, dysregulation of ZNF345 has been tentatively linked to cancer progression and neurological disorders, though mechanistic insights remain limited. Structural studies aim to map its DNA-binding specificity and interaction partners, providing groundwork for therapeutic targeting. Current investigations emphasize its potential as a biomarker or modulator in disease pathways, though functional validation in vivo is ongoing.
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