| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF33A |
| Uniprot No | Q06730 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-811 aa |
| 活性数据 | MFQVEQKSQESVSFKDVTVGFTQEEWQHLDPSQRALYRDVMLENYSNLVSVGYCVHKPEVIFRLQQGEEPWKQEEEFPSQSFPEVWTADHLKERSQENQSKHLWEVVFINNEMLTKEQGDVIGIPFNVDVSSFPSRKMFCQCDSCGMSFNTVSELVISKINYLGKKSDEFNACGKLLLNIKHDETHTQEKNEVLKNRNTLSHHEETLQHEKIQTLEHNFEYSICQETLLEKAVFNTQKRENAEENNCDYNEFGRTLCDSSSLLFHQISPSRDNHYEFSDCEKFLCVKSTLSKPHGVSMKHYDCGESGNNFRRKLCLSHLQKGDKGEKHFECNECGKAFWEKSHLTRHQRVHTGQKPFQCNECEKAFWDKSNLTKHQRSHTGEKPFECNECGKAFSHKSALTLHQRTHTGEKPYQCNACGKTFCQKSDLTKHQRTHTGLKPYECYECGKSFRVTSHLKVHQRTHTGEKPFECLECGKSFSEKSNLTQHQRIHIGDKSYECNACGKTFYHKSLLTRHQIIHTGWKPYECYECGKTFCLKSDLTVHQRTHTGQKPFACPECGKFFSHKSTLSQHYRTHTGEKPYECHECGKIFYNKSYLTKHNRTHTGEKPYECNECGKAFYQKSQLTQHQRIHIGEKPYKCNECGKAFCHKSALIVHQRTHTQEKPYKCNECGKSFCVKSGLIFHERKHTGEKPYECNECGKFFRHKSSLTVHHRAHTGEKSCQCNECGKIFYRKSELAQHQRSHTGEKPYECNTCRKTFSQKSNLIVHQRRHIGENLMNEMDIRNFQPQVSLHNASEYSHCGESPDDILNVQ |
| 分子量 | 89.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF33A蛋白的3篇代表性参考文献(内容为模拟撰写，供参考)：
1. **文献名称**：Structural and functional analysis of the zinc finger protein ZNF33A
**作者**：Smith J, et al.
**摘要**：本研究首次报道了重组人ZNF33A蛋白在大肠杆菌中的高效表达与纯化，证实其C2H2型锌指结构域具有特异性DNA结合能力，并发现其通过KRAB结构域招募转录抑制复合物参与基因沉默。
2. **文献名称**：ZNF33A modulates cell proliferation via transcriptional repression of oncogenic targets
**作者**：Li X, et al.
**摘要**：通过哺乳动物细胞系统表达重组ZNF33A蛋白，结合ChIP-seq分析，揭示了该蛋白通过靶向调控细胞周期相关基因(如CCND1)抑制肿瘤细胞生长的分子机制。
3. **文献名称**：Proteomic characterization of ZNF33A-interacting complexes in human cells
**作者**：Garcia-Ruiz C, et al.
**摘要**：采用亲和纯化-质谱技术对重组Flag标签ZNF33A蛋白的相互作用网络进行系统性分析，发现其与KAP1、SETDB1等表观遗传调控因子形成复合物，提示其在染色质重塑中的功能。
(注：真实文献需通过PubMed/Google Scholar等平台检索，上述案例为领域相关研究方向的整合描述)
Recombinant human ZNF33A (Zinc Finger Protein 33A) is a engineered version of a naturally occurring transcription factor belonging to the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF33A, also known as KOX31. is encoded by the ZNF33A gene located on human chromosome 19 and is characterized by multiple C2H2-type zinc finger motifs that mediate sequence-specific DNA binding. These zinc finger domains, combined with its KRAB domain, enable ZNF33A to regulate gene expression by recruiting chromatin-modifying complexes, thereby influencing transcriptional repression and epigenetic silencing.
As a member of the largest transcription factor family in humans, ZNF33A is implicated in diverse cellular processes, including cell differentiation, proliferation, and embryonic development. Its recombinant form is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) to ensure high purity and activity for experimental applications. Researchers utilize recombinant ZNF33A to study its DNA-binding specificity, interaction partners, and regulatory roles in diseases such as cancers or neurological disorders.
Studies on ZNF33A also contribute to understanding the evolutionary diversification of KRAB-zinc finger proteins, which play critical roles in genome defense against transposable elements. Ongoing research aims to elucidate its target genes, signaling pathways, and potential therapeutic applications in gene regulation. Quality-controlled batches are validated via techniques like SDS-PAGE, Western blotting, and functional assays to ensure batch-to-batch consistency.
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