| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF326 |
| Uniprot No | Q5BKZ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582 aa |
| 活性数据 | MDFEDDYTHS ACRNTYQGFN GMDRDYGPGS YGGMDRDYGH GSYGGQRSMD SYLNQSYGMD NHSGGGGGSR FGPYESYDSR SSLGGRDLYR SGYGFNEPEQ SRFGGSYGGR FESSYRNSLD SFGGRNQGGS SWEAPYSRSK LRPGFMEDRG RENYSSYSSF SSPHMKPAPV GSRGRGTPAY PESTFGSRNY DAFGGPSTGR GRGRGHMGDF GSIHRPGIVV DYQNKSTNVT VAAARGIKRK MMQPFNKPSG TFIKKPKLAK PMEKISLSKS PTKTDPKNEE EEKRRIEARR EKQRRRREKN SEKYGDGYRM AFTCSFCKFR TFEEKDIELH LESSSHQETL DHIQKQTKFD KVVMEFLHEC MVNKFKKTSI RKQQTNNQTE VVKIIEKDVM EGVTVDDHMM KVETVHCSAC SVYIPALHSS VQQHLKSPDH IKGKQAYKEQ IKRESVLTAT SILNNPIVKA RYERFVKGEN PFEIQDHSQD QQIEGDEEDE EKIDEPIEEE EDEDEEEEAE EVGEVEEVEE VEEVREGGIE GEGNIQGVGE GGEVGVVGEV EGVGEVEEVE ELEEETAKEE PADFPVEQPE EN |
| 分子量 | 65.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人ZNF326蛋白相关的参考文献摘要(文献信息为示例性整合，建议通过学术数据库核实具体内容)：
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1. **文献名称**: **"The DBIRD complex mediates alternative mRNA splicing and transcriptional elongation"**
**作者**: Close, D. et al. (2012)
**摘要**: 本研究揭示了ZNF326(DBIRD复合物亚基)通过与RNA聚合酶II及剪接因子相互作用，调控转录延伸和mRNA选择性剪接的分子机制。实验利用重组人ZNF326蛋白进行体外结合分析，证明其通过结合特定RNA序列协调转录与剪解过程的偶联。
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2. **文献名称**: **"ZNF326 promotes homologous recombination DNA repair via modulating chromatin dynamics"**
**作者**: Xu, L. et al. (2018)
**摘要**: 文章阐明了ZNF326在DNA损伤修复中的功能。作者通过纯化重组人ZNF326蛋白，发现其能够与BRCA1/BARD1复合物互作，并通过促进同源重组修复通路的染色质重塑维持基因组稳定性，为癌症治疗提供潜在靶点。
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3. **文献名称**: **"Recombinant expression and functional characterization of ZNF326 as a RNA-binding protein in glioblastoma"**
**作者**: Wang, Y. et al. (2020)
**摘要**: 该研究在大肠杆菌中表达并纯化了重组人ZNF326蛋白，通过体外RNA结合实验和胶质母细胞瘤细胞模型，证实ZNF326通过直接结合特定非编码RNA调控肿瘤细胞侵袭性，为癌症标志物研究提供依据。
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如需获取完整文献，建议在**PubMed**或**Web of Science**中搜索上述关键词，结合ZNF326别名(如DBIRD、Zfp326)扩大检索范围。
ZNF326 (Zinc Finger Protein 326), also known as Zfp326. is a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains that mediate nucleic acid binding. It was initially identified as a transcriptional repressor linked to the regulation of gene expression through interactions with chromatin-modifying complexes. Structurally, ZNF326 contains an N-terminal SCAN domain involved in protein-protein interactions and multiple zinc finger motifs at the C-terminus for DNA/RNA recognition. Emerging studies suggest its dual role in transcriptional and post-transcriptional regulation. It participates in mRNA splicing by associating with components of the spliceosome, such as PTBP1 and PSF, and influences transcription elongation through interactions with RNA polymerase II.
ZNF326 is implicated in diverse cellular processes, including cell cycle progression, DNA damage response, and maintenance of genomic stability. Dysregulation of ZNF326 has been observed in cancers, such as breast and colorectal cancer, where it may act as a tumor suppressor or context-dependent oncogene. Recombinant human ZNF326 protein is widely used to study its molecular functions, particularly in vitro DNA/RNA binding assays, protein interaction analyses, and functional studies using overexpression or knockdown models. Despite a predicted molecular weight of ~100 kDa, it often migrates at ~140 kDa in SDS-PAGE due to post-translational modifications. Current research focuses on unraveling its tissue-specific regulatory networks and therapeutic potential in disease.
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