| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF322A |
| Uniprot No | Q6U7Q0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402 aa |
| 活性数据 | MYTSEEKCNQ RTQKRKIYNV CPRKGKKIFI HMHEIIQIDG HIYQCLECKQ NFCENLALIM CERTHTGEKP YKCDMCEKTF VQSSDLTSHQ RIHNYEKPYK CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS DLLVHQRSHT GEKPYLCSEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK AFSGKSDLIS HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK CGACEKCFGQ KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT HTFKCLEYEK SFNCSSDLIV HQRIHMEEKP HQWSACESGF LLGMDFVAQQ KMRTQTEELH YKYTVCDKSF HQSSALLQHQ TVHIGEKPFV CNVSEKGLEL SPPHASEASQ MS |
| 分子量 | 46.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF322A蛋白的3篇参考文献的简要整理(基于现有领域知识模拟，非真实文献)：
---
1. **文献名称**：*ZNF322A promotes cell proliferation and invasion via transcriptional activation of PI3K/AKT signaling in lung adenocarcinoma*
**作者**：Chen L, et al.
**摘要**：研究通过重组ZNF322A蛋白表达，发现其通过直接结合靶基因启动子激活PI3K/AKT通路，促进肺癌细胞增殖、迁移及侵袭，提示其作为肺癌治疗潜在靶点。
2. **文献名称**：*Recombinant ZNF322A protein regulates epithelial-mesenchymal transition by modulating E-cadherin expression*
**作者**：Wang Y, et al.
**摘要**：利用重组ZNF322A蛋白体外实验，揭示其通过抑制E-cadherin转录诱导上皮间质转化(EMT)，促进肿瘤细胞转移，机制涉及与组蛋白修饰酶HDAC3的相互作用。
3. **文献名称**：*Structural and functional characterization of the recombinant ZNF322A DNA-binding domain*
**作者**：Zhang R, et al.
**摘要**：通过原核系统表达并纯化ZNF322A的锌指结构域，结合晶体结构分析和DNA结合实验，阐明其特异性识别GC-rich序列的分子机制，为设计靶向抑制剂提供结构基础。
---
**注**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“ZNF322A recombinant protein”获取。建议结合具体研究方向(如癌症、转录调控)筛选高质量期刊论文。
ZNF322A, a member of the zinc finger protein family, is a transcription factor encoded by the human ZNF322A gene. Structurally, it contains multiple C2H2-type zinc finger domains, which enable sequence-specific DNA binding and interactions with other proteins. This protein is implicated in transcriptional regulation, influencing processes like cell proliferation, differentiation, and apoptosis. Studies suggest its involvement in critical signaling pathways, including MAPK/ERK and Wnt, impacting embryonic development and tissue homeostasis.
Dysregulation of ZNF322A has been linked to cancers, though its role appears context-dependent. For example, it may act as an oncogene in lung cancer by promoting cell invasion and metastasis but exhibit tumor-suppressive functions in hepatocellular carcinoma. Such duality underscores its complex regulatory mechanisms. Recombinant human ZNF322A protein, produced via bacterial or mammalian expression systems, is widely utilized in biochemical assays (e.g., DNA-binding studies), cellular functional analyses (e.g., CRISPR/Cas9 rescue experiments), and drug discovery screens targeting its activity.
Ongoing research aims to clarify its precise molecular interactions and therapeutic potential. Despite conflicting reports on its roles in different cancers, ZNF322A remains a promising candidate for understanding transcriptional networks and developing targeted cancer therapies.
×
