| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF333 |
| Uniprot No | Q96JL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665 aa |
| 活性数据 | MESVTFEDVA VEFIQEWALL DSARRSLCKY RMLDQCRTLA SRGTPPCKPS CVSQLGQRAE PKATERGILR ATGVAWESQL KPEELPSMQD LLEEASSRDM QMGPGLFLRM QLVPSIEERE TPLTREDRPA LQEPPWSLGC TGLKAAMQIQ RVVIPVPTLG HRNPWVARDS AVPARDPAWL QEDKVEEEAM APGLPTACSQ EPVTFADVAV VFTPEEWVFL DSTQRSLYRD VMLENYRNLA SVADQLCKPN ALSYLEERGE QWTTDRGVLS DTCAEPQCQP QEAIPSQDTF TEILSIDVKG EQPQPGEKLY KYNELEKPFN SIEPLFQYQR IHAGEASCEC QEIRNSFFQS AHLIVPEKIR SGDKSYACNK CEKSFRYSSD LIRHEKTHTA EKCFDCQECG QAFKYSSNLR RHMRTHTGEK PFECSQCGKT FTRNFNLILH QRNHTGEKPY ECKDCGKAFN QPSSLRSHVR THTGEKPFEC SQCGKAFREH SSLKTHLRTH TREKPYECNQ CGKPFRTSTH LNVHKRIHTG EKLYECATCG QVLSRLSTLK SHMRTHTGEK PYVCQECGRA FSEPSSLRKH ARTHSGKKPY ACQECGRAFG QSSHLIVHVR THSAGRPYQC NQCEKAFRHS SSLTVHKRTH VGRETIRNGS LPLSMSHPYC GPLAN |
| 分子量 | 75.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF333蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
1. **文献名称**: "ZNF333 Recombinant Protein Expression and Its Role in Transcriptional Repression"
**作者**: Li X, Wang Y, et al.
**摘要**: 该研究利用大肠杆菌系统成功表达并纯化了重组人ZNF333蛋白，发现其通过结合特定DNA序列抑制下游基因的转录活性，提示其在表观遗传调控中的作用。
2. **文献名称**: "Structural Analysis of ZNF333 Zinc Finger Motif and Interaction with DNA"
**作者**: Zhang R, Chen H, et al.
**摘要**: 通过X射线晶体学解析了ZNF333蛋白的锌指结构域，证实其与DNA靶点的特异性结合模式，为设计靶向ZNF333的分子工具提供结构基础。
3. **文献名称**: "ZNF33B Recombinant Protein Suppresses Tumor Growth in Colorectal Cancer Models"
**作者**: Kim S, Park J, et al.
**摘要**: 实验表明，重组ZNF333蛋白通过抑制Wnt/β-catenin通路显著降低结直肠癌细胞增殖，提示其作为肿瘤抑制因子的潜在治疗价值。
4. **文献名称**: "Functional Screening of ZNF333 Interaction Partners Using Yeast Two-Hybrid System"
**作者**: Tanaka K, Sato M, et al.
**摘要**: 研究利用重组ZNF333蛋白筛选到多个互作蛋白(如HDAC1和CTBP2)，表明ZNF333可能参与染色质修饰复合物的组装，调节基因沉默。
**注意**：以上文献为模拟内容，实际研究中请通过PubMed、Web of Science等平台检索最新文献，并使用正确引用格式(如APA、MLA等)。ZNF333的研究可能涉及基因调控、疾病机制等方向，建议结合具体研究目的筛选文献。
Zinc finger protein 333 (ZNF333), also known as ZPR1 or ZKSCAN16. is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZNF) family. These proteins are characterized by tandem arrays of C2H2-type zinc finger motifs that enable sequence-specific DNA binding, functioning primarily as transcription regulators. ZNF333 is thought to play roles in cell differentiation, development, and apoptosis by modulating target gene expression through interactions with chromatin-modifying complexes.
The human ZNF333 gene is located on chromosome 19q13.43 and encodes a 510-amino-acid protein containing a KRAB domain (involved in transcriptional repression) and eight zinc finger repeats. Dysregulation of ZNF333 has been implicated in several pathologies, including cancers such as colorectal carcinoma and hepatocellular carcinoma, where it may act as a tumor suppressor or promoter depending on cellular context. Emerging studies also link ZNF333 to neurological disorders and autoimmune diseases, suggesting broader functional versatility.
Recombinant human ZNF333 protein is typically produced using expression systems like *E. coli* or mammalian cells for biochemical and functional studies. Its recombinant form facilitates research into DNA-protein interactions, transcriptional networks, and potential therapeutic targeting. Despite progress, the full spectrum of ZNF333's biological roles and molecular mechanisms remains under investigation, particularly its interplay with signaling pathways like Wnt and TGF-β.
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