| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF324 |
| Uniprot No | O75467 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553 aa |
| 活性数据 | MAFEDVAVYF SQEEWGLLDT AQRALYRRVM LDNFALVASL GLSTSRPRVV IQLERGEEPW VPSGTDTTLS RTTYRRRNPG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVAGACHSVK SLQRQRGASP SRERKPTGVS VIYWERLLLG SGSGQASVSL RLTSPLRPPE GVRLREKTLT EHALLGRQPR TPERQKPCAQ EVPGRTFGSA QDLEAAGGRG HHRMGAVWQE PHRLLGGQEP STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECAQC GKAFSQTSHL TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFKHQRVHT GEKPFACPQC GRAFSHSSNL TQHQLLHTGE RPFRCVDCGK AFAKGAVLLS HRRIHTGEKP FVCTQCGRAF RERPALFHHQ RIHTGEKTVR RSRASLHPQA RSVAGASSEG APAKETEPTP ASGPAAVSQP AEV |
| 分子量 | 61.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《Recombinant expression and functional characterization of human ZNF324 in HEK293 cells》**
*作者：Zhang et al., 2020*
**摘要**：该研究成功克隆并在HEK293细胞中表达了重组人ZNF324蛋白，纯化后通过体外实验证实其具有特异性DNA结合能力，提示其在基因转录调控中的潜在作用。
2. **《ZNF324 modulates epigenetic silencing of tumor suppressor genes in cancer progression》**
*作者：Lee & Kim, 2018*
**摘要**：文章探讨ZNF324蛋白与染色质重塑复合物的相互作用，发现其通过招募组蛋白去乙酰化酶(HDACs)促进肿瘤抑制基因的沉默，与多种癌症的恶性表型相关。
3. **《Structural insights into the zinc finger domains of ZNF324 and their DNA recognition motifs》**
*作者：Smith et al., 2021*
**摘要**：通过X射线晶体学解析ZNF324蛋白的锌指结构域，揭示了其与特定DNA序列(如GC-rich区域)结合的分子机制，为设计靶向ZNF324的药物奠定结构基础。
4. **《Role of ZNF324 in neurodevelopment: CRISPR-based functional analysis》**
*作者：Chen et al., 2019*
**摘要**：利用CRISPR敲除技术研究ZNF324在神经元分化中的功能，发现其缺失导致神经突触相关基因(如SYN1)表达下调，提示其在神经发育障碍中的潜在作用。
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注：以上文献信息及作者为虚构示例，实际研究中建议通过PubMed或专业数据库查询具体文献。
ZNF324. a member of the zinc finger protein family, is characterized by conserved Cys2-His2 (C2H2) zinc finger motifs that enable sequence-specific binding to DNA or RNA. This protein plays a role in transcriptional regulation, influencing cellular processes such as differentiation, proliferation, and apoptosis. The human ZNF324 gene is located on chromosome 19 (19p13.2) and encodes a protein containing multiple tandem zinc finger domains, which are critical for its function as a putative transcription factor. While its exact physiological role remains under investigation, studies suggest involvement in cancer progression, neurodegenerative disorders, and immune responses due to its regulatory effects on gene networks.
Recombinant human ZNF324 protein, produced via expression systems like *E. coli* or mammalian cells, retains the DNA-binding capacity of its native counterpart. This engineered protein facilitates structural and functional studies, including chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and protein interaction mapping. Researchers leverage recombinant ZNF324 to explore its interaction with target genes, pathological mechanisms in diseases, and potential therapeutic applications. Despite progress, the full spectrum of its biological targets and disease relevance requires further elucidation through high-throughput omics approaches and *in vivo* models.
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