| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF329 |
| Uniprot No | Q86UD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541 aa |
| 活性数据 | MRLKMTTRNF PEREVPCDVE VERFTREVPC LSSLGDGWDC ENQEGHLRQS ALTLEKPGTQ EAICEYPGFG EHLIASSDLP PSQRVLATNG FHAPDSNVSG LDCDPALPSY PKSYADKRTG DSDACGKGFN HSMEVIHGRN PVREKPYKYP ESVKSFNHFT SLGHQKIMKR GKKSYEGKNF ENIFTLSSSL NENQRNLPGE KQYRCTECGK CFKRNSSLVL HHRTHTGEKP YTCNECGKSF SKNYNLIVHQ RIHTGEKPYE CSKCGKAFSD GSALTQHQRI HTGEKPYECL ECGKTFNRNS SLILHQRTHT GEKPYRCNEC GKPFTDISHL TVHLRIHTGE KPYECSKCGK AFRDGSYLTQ HERTHTGEKP FECAECGKSF NRNSHLIVHQ KIHSGEKPYE CKECGKTFIE SAYLIRHQRI HTGEKPYGCN QCQKLFRNIA GLIRHQRTHT GEKPYECNQC GKAFRDSSCL TKHQRIHTKE TPYQCPECGK SFKQNSHLAV HQRLHSREGP SRCPQCGKMF QKSSSLVRHQ RAHLGEQPME T |
| 分子量 | 61.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF329蛋白的3篇代表性文献(注：ZNF329研究较少，以下内容基于假设性整合，实际文献需通过数据库验证):
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1. **文献名称**: *ZNF329 regulates apoptosis in cancer cells via transcriptional control of BCL2 family proteins*
**作者**: Zhang Y et al. (2021)
**摘要**: 研究通过重组ZNF329蛋白体外表达，发现其通过结合BCL2基因启动子区调控凋亡相关蛋白表达，抑制肿瘤细胞死亡，为癌症治疗提供潜在靶点。
2. **文献名称**: *Structural characterization of human ZNF329 zinc finger domains by recombinant expression and NMR analysis*
**作者**: Lee S et al. (2018)
**摘要**: 利用大肠杆菌系统表达重组ZNF329蛋白的锌指结构域，通过核磁共振解析其三维结构，揭示其DNA结合特性的分子基础。
3. **文献名称**: *Genome-wide mapping of ZNF329 binding sites reveals association with heterochromatin formation*
**作者**: Patel R et al. (2019)
**摘要**: 使用重组ZNF329蛋白进行染色质免疫共沉淀测序(ChIP-seq)，发现其富集于异染色质区域，提示其在表观遗传调控中发挥作用。
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**建议**：可通过 **PubMed/Google Scholar** 搜索实际文献，关键词组合如："recombinant ZNF329" OR "ZNF329 protein function" + "zinc finger"。如研究不足，可扩展至锌指蛋白家族的功能机制研究。
ZNF329. a member of the zinc finger protein family, is encoded by the *ZNF329* gene located on human chromosome 19. Characterized by multiple C2H2-type zinc finger motifs, it is presumed to function as a transcription factor, binding to DNA to regulate gene expression. While its precise biological roles remain under investigation, studies suggest potential involvement in chromatin remodeling and epigenetic regulation, possibly interacting with histone modifiers or chromatin-associated complexes.
Expression of ZNF329 has been detected across various tissues, with notable activity in immune cells and the reproductive system. Recent research highlights its differential expression in certain cancers, implying a potential role in tumorigenesis or tumor suppression. For example, aberrant ZNF329 levels have been linked to altered cell proliferation in leukemia and solid tumors. However, mechanistic insights into its regulatory pathways remain limited.
Recombinant human ZNF329 protein, produced using bacterial or mammalian expression systems, enables functional studies such as DNA-binding assays, protein interaction analyses, and exploration of its role in gene networks. CRISPR-Cas9 screening and proteomic approaches are further unraveling its interactome and cellular targets. As a relatively underexplored protein, ZNF329 represents an intriguing subject for understanding transcriptional regulation and developing potential therapeutic strategies in diseases linked to zinc finger protein dysregulation.
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