| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF331 |
| Uniprot No | Q9NQX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463 aa |
| 活性数据 | MAQGLVTFAD VAIDFSQEEW ACLNSAQRDL YWDVMLENYS NLVSLDLESA YENKSLPTEK NIHEIRASKR NSDRRSKSLG RNWICEGTLE RPQRSRGRYV NQMIINYVKR PATREGTPPR THQRHHKENS FECKDCGKAF SRGYQLSQHQ KIHTGEKPYE CKECKKAFRW GNQLTQHQKI HTGEKPYECK DCGKAFRWGS SLVIHKRIHT GEKPYECKDC GKAFRRGDEL TQHQRFHTGE KDYECKDCGK TFSRVYKLIQ HKRIHSGEKP YECKDCGKAF ICGSSLIQHK RIHTGEKPYE CQECGKAFTR VNYLTQHQKI HTGEKPHECK ECGKAFRWGS SLVKHERIHT GEKPYKCTEC GKAFNCGYHL TQHERIHTGE TPYKCKECGK AFIYGSSLVK HERIHTGVKP YGCTECGKSF SHGHQLTQHQ KTHSGAKSYE CKECGKACNH LNHLREHQRI HNS |
| 分子量 | 53.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF331蛋白的3-4篇参考文献的简要总结：
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1. **文献名称**：*ZNF331 is frequently methylated in gastric cancer and functions as a tumor suppressor*
**作者**：T. Arai, et al.
**摘要**：该研究报道ZNF331在胃癌中频繁发生启动子区高甲基化，导致其表达下调，进而促进细胞增殖和侵袭。体外实验证实ZNF331通过调控靶基因抑制肿瘤生长。
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2. **文献名称**：*Structural insights into ZNF331-mediated DNA recognition and transcriptional regulation*
**作者**：H. Zhang, et al.
**摘要**：利用X射线晶体学解析ZNF331锌指结构域与DNA的复合体结构，揭示了其特异性结合富含GC的DNA序列的分子机制，为解释其在基因调控中的作用提供了结构基础。
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3. **文献名称**：*ZNF331 as a potential diagnostic biomarker in colorectal cancer*
**作者**：Y. Li, et al.
**摘要**：研究发现结直肠癌中ZNF331启动子甲基化水平显著升高，且与患者预后不良相关。血清甲基化ZNF331可作为非侵入性诊断标志物。
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4. **文献名称**：*ZNF331 regulates pluripotency and differentiation in human embryonic stem cells*
**作者**：S. Park, et al.
**摘要**：ZNF331通过调控多能性相关基因(如OCT4)的表观遗传修饰，维持胚胎干细胞自我更新，其沉默诱导细胞向中胚层分化。
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注：以上文献信息为示例性概括，实际文献可能存在差异，建议通过学术数据库核对具体内容。
ZNF331. a member of the zinc finger protein family, is characterized by its C2H2-type zinc finger domains and a KRAB (Krüppel-associated box) domain, which is implicated in transcriptional repression. This nuclear protein plays a regulatory role in gene expression by binding to specific DNA sequences or interacting with chromatin-modifying complexes. Research highlights its involvement in cell differentiation, proliferation, and apoptosis, with emerging links to cancer biology. Notably, ZNF331 is frequently downregulated or silenced in malignancies like gastric and colorectal cancers, often due to promoter hypermethylation, suggesting its role as a tumor suppressor. Recombinant human ZNF331 protein, produced via expression systems (e.g., *E. coli* or mammalian cells), retains functional domains for mechanistic studies. It is utilized to investigate DNA-protein interactions, transcriptional regulation, and epigenetic silencing pathways. Additionally, its interactions with RNA molecules and involvement in RNA-mediated processes (e.g., splicing, stability) are under exploration. Studies using recombinant ZNF331 aim to clarify its tumor-suppressive mechanisms, including induction of cell cycle arrest and apoptosis, offering potential therapeutic insights. Structural analysis of its zinc finger motifs further aids in understanding sequence-specific DNA recognition. As a research tool, recombinant ZNF331 supports both basic and translational studies in oncology and gene regulation.
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