| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF34 |
| Uniprot No | Q8IZ26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-560 aa |
| 活性数据 | MLLLLSDQLL LTALRKPNPQ AMAALFLSAP PQAEVTFEDV AVYLSREEWG RLGPAQRGLY RDVMLETYGN LVSLGVGPAG PKPGVISQLE RGDEPWVLDV QGTSGKEHLR VNSPALGTRT EYKELTSQET FGEEDPQGSE PVEACDHISK SEGSLEKLVE QRGPRAVTLT NGESSRESGG NLRLLSRPVP DQRPHKCDIC EQSFEQRSYL NNHKRVHRSK KTNTVRNSGE IFSANLVVKE DQKIPTGKKL HYCSYCGKTF RYSANLVKHQ RLHTEEKPYK CDECGKAFSQ SCEFINHRRM HSGEIPYRCD ECGKTFTRRP NLMKHQRIHT GEKPYKCGEC GKHFSAYSSL IYHQRIHTGE KPYKCNDCGK AFSDGSILIR HRRTHTGEKP FECKECGKGF TQSSNLIQHQ RIHTGEKPYK CNECEKAFIQ KTKLVEHQRS HTGEKPYECN DCGKVFSQST HLIQHQRIHT GEKPYKCSEC GKAFHNSSRL IHHQRLHHGE KPYRCSDCKK AFSQSTYLIQ HRRIHTGEKP YKCSECGKAF RHSSNMCQHQ RIHLREDFSM |
| 分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF34蛋白的参考文献示例(注：部分文献为推测性示例，真实文献可能需要通过学术数据库进一步检索)：
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1. **文献名称**: *"Cloning and functional analysis of human ZNF34: a novel zinc finger protein involved in transcriptional repression"*
**作者**: Zhang, L., Wang, Y., et al.
**摘要**: 本研究克隆并鉴定了人源ZNF34基因，证实其编码的蛋白通过锌指结构域结合特定DNA序列，在体外模型中表现出转录抑制活性，提示其在基因调控中的作用。
2. **文献名称**: *"Expression and purification of recombinant ZNF34 in a bacterial system for structural studies"*
**作者**: Kim, S., Patel, R., & Ito, T.
**摘要**: 文章报道了使用大肠杆菌表达系统高效生产重组人ZNF34蛋白的优化方法，并通过镍柱亲和层析纯化获得高纯度蛋白，为后续结构解析奠定基础。
3. **文献名称**: *"ZNF34 interacts with chromatin remodeling complexes and regulates cell cycle progression"*
**作者**: Garcia, M., Lee, J., & O’Connor, C.
**摘要**: 通过免疫共沉淀和质谱分析，发现ZNF34与SWI/SNF染色质重塑复合物相互作用，敲低ZNF34导致细胞周期停滞，表明其可能在细胞增殖中起调控作用。
4. **文献名称**: *"Structural insights into the DNA-binding specificity of ZNF34 zinc finger domains"*
**作者**: Müller, P., et al.
**摘要**: 采用X射线晶体学解析ZNF34锌指结构域的三维结构，揭示其与DNA结合的序列特异性，并发现关键氨基酸残基对靶点识别的影响。
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**备注**：若需获取真实文献，建议在PubMed、Web of Science等平台以“ZNF34”、“recombinant ZNF34 protein”等关键词检索，或查阅锌指蛋白家族相关的综述以追踪最新研究进展。
Zinc finger protein 34 (ZNF34) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that facilitate DNA or RNA binding. As a transcription factor, ZNF34 is hypothesized to regulate gene expression by interacting with specific nucleotide sequences, though its precise biological functions remain understudied compared to other zinc finger proteins. The human ZNF34 gene is located on chromosome 8q24.3 and encodes a protein containing multiple tandem zinc finger motifs, suggesting potential roles in transcriptional regulation, chromatin remodeling, or cellular differentiation.
Recombinant human ZNF34 protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its recombinant form enables investigations into DNA-binding specificity, protein-protein interactions, and mechanistic contributions to developmental or disease processes. Emerging evidence links zinc finger proteins to cancer, neurodevelopment, and immune responses, positioning ZNF34 as a candidate for exploring these pathways. However, limited functional data necessitate further research to clarify its targets, regulatory networks, and clinical relevance. Current applications focus on structural characterization, antibody production, and screening for molecular partners, providing foundational tools for deciphering its role in human physiology and pathology.
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