| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF354C |
| Uniprot No | Q86Y25 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-554 aa |
| 活性数据 | MAVDLLSAQE PVTFRDVAVF FSQDEWLHLD SAQRALYREV MLENYSSLVS LGIPFSMPKL IHQLQQGEDP CMVEREVPSD TRLGFKTWLE TEALPHRQDI FIEETSQGMV KKESIKDGHW DINFEEAVEF ESEIEEEQEK KPLRQMIDSH EKTISEDGNH TSLELGKSLF TNTALVTQQS VPIERIPNMY YTFGKDFKQN FDLMKCFQIY PGGKPHICNE CGKSFKQNLH LIEHQRIHTG EKPYKCNECE KTFSHRSSLL SHQRIHTGEK PYKCNECEKA FSNSSTLIKH LRVHTGEKPY RCRECGKAFS QCSTLTVHQR IHTGEKLYKC GECEKAFNCR AKLHRHQRIH TGEKPYKCSE CGKGYSQFTS LAEHQRFHTG EQLYTCLECG RTFTRIVTLI EHQRIHTGQK PYQCNECEKA FNQYSSFNEH RKIHTGEKLY TCEECGKAFG CKSNLYRHQR IHTGEKPYQC NQCGKAFSQY SFLTEHERIH TGEKLYKCME CGKAYSYRSN LCRHKKVHTK EKLYKWKEYG KPFICSSSLT QYQRFFKGDK AYEV |
| 分子量 | 64.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF354C蛋白的参考文献示例(部分为模拟内容，仅供参考格式)：
---
1. **标题**：*"Recombinant human ZNF354C protein binds to promoter regions of tumor suppressor genes in vitro"*
**作者**：Smith A. et al. (2018)
**摘要**：通过大肠杆菌重组表达并纯化ZNF354C蛋白，发现其通过锌指结构域与多种肿瘤抑制基因(如p53)的启动子结合，可能参与转录调控及癌症发生。
2. **标题**：*"Structural characterization of recombinant ZNF354C and its role in epigenetic silencing"*
**作者**：Zhang L. et al. (2020)
**摘要**：解析重组ZNF354C蛋白的晶体结构，揭示其与DNA甲基化转移酶的相互作用，并证明其通过招募表观遗传修饰复合物调控基因沉默。
3. **标题**：*"Functional analysis of ZNF354C in neuronal differentiation using recombinant protein"*
**作者**：Chen H. et al. (2021)
**摘要**：利用重组ZNF354C蛋白处理神经干细胞，发现其促进神经元分化相关基因(如NeuroD1)表达，提示其在神经系统发育中的潜在作用。
4. **标题**：*"Recombinant ZNF354C inhibits cell proliferation by modulating the Wnt/β-catenin pathway"*
**作者**：Johnson R. et al. (2019)
**摘要**：体外实验表明，重组ZNF354C蛋白通过干扰β-catenin与TCF4的结合抑制Wnt通路活性，从而抑制结直肠癌细胞增殖。
---
**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的文献。
Recombinant human ZNF354C protein is a zinc finger-containing transcription factor encoded by the ZNF354C gene, located on chromosome 5 in humans. As a member of the C2H2 zinc finger protein family, it features multiple tandem zinc finger motifs at its C-terminus, enabling sequence-specific DNA binding, and a conserved N-terminal POZ/BTB domain that mediates protein-protein interactions. ZNF354C is implicated in transcriptional regulation, chromatin remodeling, and epigenetic silencing, particularly through recruitment of corepressors or histone-modifying enzymes like HDACs or methyltransferases to repress target gene expression. Studies suggest its role in cellular processes such as differentiation, proliferation, and apoptosis, with potential links to cancer pathogenesis, as it may suppress oncogenes or modulate tumor suppressors. Additionally, it has been associated with neurological functions, possibly influencing neuronal development or synaptic plasticity. Recombinant ZNF354C, typically produced in bacterial or mammalian expression systems, serves as a tool for studying DNA-protein interactions, transcriptional networks, and disease mechanisms. Despite advances, the full scope of its biological roles and regulatory targets remains under investigation, highlighting its significance in both basic research and therapeutic target exploration.
×
