| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF418 |
| Uniprot No | Q8TF45 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676 aa |
| 活性数据 | MQGTVAFEDV AVNFSQEEWS LLSEVQRCLY HDVMLENWVL ISSLGCWCGS EDEEAPSKKS ISIQRVSQVS TPGAGVSPKK AHSCEMCGAI LGDILHLADH QGTHHKQKLH RCEAWGNKLY DSSNRPHQNQ YLGEKPYRSS VEEALFVKRC KFHVSEESSI FIQSGKDFLP SSGLLLQEAT HTGEKSNSKP ECESPFQWGD THYSCGECMK HSSTKHVFVQ QQRLPSREEC YCWECGKSFS KYDSVSNHQR VHTGKRPYEC GECGKSFSHK GSLVQHQRVH TGKRPYECGE CGKSFSHKGS LVQHQRVHTG ERPYECGECG KSFSQNGTLI KHQRVHTGER PYECEECGKC FTQKGNLIQH QRGHTSERPY ECEECGKCFS QKGTLTEHHR VHTRERPYEC GECGKSFSRK GHLRNHQRGH TGERPYECGE CGKSFSRKGN LIQHQRSHTG ERPYECRECR KLFRGKSHLI EHQRVHTGER PYECNECGKS FQDSSGFRVH QRVHTGEKPF ECSECGKSFP QSCSLLRHRR VHTGERPYEC GECGKSFHQS SSLLRHQKTH TAERPYECRE CGKFFSSLLE HRRVHTGERP YECRECGKTF TRRSAHFKHQ RLHTRGKPYE CSECGKSFAE TFSLTEHRRV HTGERPYECS ECGKSFHRSS SLLRHQRVHT ERSPYK |
| 分子量 | 77.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF418蛋白的模拟参考文献示例，基于假设性研究内容整理而成：
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1. **文献名称**: *"Recombinant Human ZNF418 Protein: Expression, Purification, and DNA-Binding Characterization"*
**作者**: Zhang, Y. et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化重组人ZNF418蛋白，通过凝胶迁移实验(EMSA)证实其特异性结合靶DNA序列，为后续功能研究奠定基础。
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2. **文献名称**: *"ZNF418 as a Transcriptional Repressor in Neural Stem Cell Differentiation"*
**作者**: Li, H. et al.
**摘要**: 研究揭示了ZNF418通过KRAB结构域招募组蛋白去乙酰化酶(HDACs)，抑制神经元分化相关基因的转录，利用重组ZNF418蛋白验证其对干细胞分化的调控作用。
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3. **文献名称**: *"Structural Insights into ZNF418's Zinc Finger Motif and Implications for Genetic Disorders"*
**作者**: Wang, X. & Chen, J.
**摘要**: 通过X射线晶体学解析ZNF418的锌指结构域，发现其突变体与智力障碍相关，重组蛋白实验显示突变导致DNA结合能力显著下降。
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4. **文献名称**: *"ZNF418-Mediated Regulation of Insulin Signaling in Type 2 Diabetes"*
**作者**: Kim, S. et al.
**摘要**: 在小鼠模型中，重组ZNF418蛋白过表达改善了胰岛素抵抗，机制研究表明其通过抑制炎症通路(如NF-κB)增强胰岛素敏感性。
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**备注**：以上文献为假设性示例，实际研究需参考真实发表的学术论文。建议通过PubMed或Google Scholar检索“ZNF418”、“recombinant ZNF418”等关键词获取最新进展。
Zinc finger protein 418 (ZNF418) is a member of the zinc finger protein family, characterized by conserved Cys2His2 (C2H2)-type zinc finger domains that facilitate sequence-specific DNA binding. As a transcription factor, ZNF418 is implicated in the regulation of gene expression, particularly during development and cellular differentiation. It is predominantly expressed in the nervous system and hematopoietic tissues, suggesting roles in neurodevelopment and blood cell maturation. Studies have linked ZNF418 to the modulation of signaling pathways, including those involved in stress responses and apoptosis, though its precise molecular targets remain under investigation.
Recombinant human ZNF418 protein, produced via heterologous expression systems such as *E. coli* or mammalian cells, enables functional and structural studies. Its recombinant form is often tagged (e.g., His-tag) for purification and detection, retaining DNA-binding activity for *in vitro* assays like electrophoretic mobility shift assays (EMSAs). Research highlights its potential involvement in pathologies; for instance, dysregulation of ZNF418 has been observed in neurological disorders and certain cancers, hinting at its dual roles as a tumor suppressor or oncogenic driver depending on context. Deciphering ZNF418's interactions and regulatory networks could advance understanding of cellular differentiation mechanisms and disease pathogenesis, offering avenues for therapeutic targeting. Further work is needed to elucidate its tissue-specific functions and clinical relevance.
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