| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF426 |
| Uniprot No | Q9BUY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-554 aa |
| 活性数据 | MAAADLSHGH YLSGDPVCLH EEKTPAGRIV ADCLTDCYQD SVTFDDVAVD FTQEEWTLLD STQRSLYSDV MLENYKNLAT VGGQIIKPSL ISWLEQEESR TVQGGVLQGW EMRLETQWSI LQQDFLRGQT SIGIQLEGKH NGRELCDCEQ CGEVFSEHSC LKTHVRTQST GNTHDCNQYG KDFLTLCEKT STGEKLSEFN QSEKIFSLTP NIVYQRTSTQ EKSFECSHCG KSFINESYLQ AHMRTHNGEK LYEWRNYGPG FIDSTSLSVL IETLNAKKPY KCKECGKGYR YPAYLSIHMR THTGEKPYEC KECGKAFNYS NSFQIHGRTH TGEKPYVCKE CGKAFTQYSG LSMHVRSHSG DKPYECKECG KSFLTSSRLI QHIRTHTGEK PFVCVECGKA FAVSSNLSGH LRTHTEEKAC ECKICGKVFG YPSCLNNHMR THSAQKPYTC KECGKAFNYS THLKIHMRIH TGEKPYECKQ CGKAFSHSSS FQIHERTHTG EKPYECKECG KAFTCSSSFR IHEKTHTEEK PYKCQQCGKA YSHPRSLRRH EQIH |
| 分子量 | 63.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于重组人ZNF426蛋白的参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**：*Cloning and Expression of Recombinant Human ZNF426 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究利用大肠杆菌表达系统成功克隆并纯化了重组人ZNF426蛋白。通过优化诱导条件提高了蛋白可溶性，并验证其与DNA结合的活性，为后续功能研究提供工具。
2. **标题**：*Structural Analysis of ZNF426 Reveals a Novel Zinc Finger Motif*
**作者**：Lee H, et al.
**摘要**：通过X射线晶体学解析ZNF426的三维结构，发现其锌指结构域具有独特的氨基酸排列，可能参与特异性识别靶基因启动子区域。
3. **标题**：*Functional Screening of ZNF426 in Transcriptional Regulation*
**作者**：Zhang R, et al.
**摘要**：利用双荧光素酶报告系统证明重组ZNF426可抑制特定癌基因(如MYC)的转录活性，提示其可能作为肿瘤抑制因子发挥作用。
4. **标题**：*Interactome Profiling of ZNF426 Using Affinity Purification-Mass Spectrometry*
**作者**：Kumar S, et al.
**摘要**：通过亲和纯化-质谱技术鉴定了ZNF426的相互作用蛋白网络，包括组蛋白修饰酶(如HDAC1)，表明其在表观遗传调控中的潜在作用。
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注意：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索确认。
Zinc finger protein 426 (ZNF426) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which represents one of the largest groups of transcription regulators in humans. These proteins are characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, coupled with a KRAB domain typically involved in transcriptional repression through interactions with co-repressors. ZNF426 is encoded by the human gene located on chromosome 19q13.43. though its precise biological functions remain less characterized compared to other well-studied zinc finger proteins.
As a putative transcriptional regulator, ZNF426 is hypothesized to play roles in developmental processes, cell differentiation, or chromatin remodeling, consistent with the general functions of KRAB-ZNF proteins in controlling gene expression networks. Recombinant human ZNF426 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables in vitro studies to map its DNA-binding specificity, protein interaction partners, and structural features. Current research focuses on elucidating its target genes, regulatory mechanisms, and potential associations with diseases, though substantial knowledge gaps persist. The availability of recombinant ZNF426 facilitates biochemical assays, antibody production, and functional studies to advance understanding of its role in cellular processes and human pathophysiology.
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