| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTL1 |
| Uniprot No | Q8WWI5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-657aa |
| 氨基酸序列 | MGCCSSASSAAQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLVSGYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGVMTSKEIILGLCLLSLVLSMILMVIIRYISRVLVWILTILVILGSLGGTGVLWWLYAKQRRSPKETVTPEQLQIAEDNLRALLIYAISATVFTVILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWVYWIMTLLFLGTTGSPVQNEQGFVEFKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDKRNLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCVLKSCICCLWCLEKCLNYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVATINTVGDFMLFLGKVLIVCSTGLAGIMLLNYQQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGREFYMDKVLMEFVENSRKAMKEAGKGGVADSRELKPMASGASSA |
| 预测分子量 | 73,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CTL1重组蛋白相关的代表性文献(基于公开研究总结，非真实文献)：
1. **文献名称**：*Molecular characterization of human CTL1/SLC44A1 recombinant protein in choline transport*
**作者**：Inoue K, et al.
**摘要**：该研究通过构建人源CTL1重组蛋白表达系统，验证其在HEK293细胞中的胆碱转运功能，揭示其pH依赖性及对胆碱类似物的特异性结合能力。
2. **文献名称**：*Functional analysis of recombinant CTL1 in the blood-brain barrier*
**作者**：Michel V, et al.
**摘要**：利用重组CTL1蛋白模型，证明其在血脑屏障内皮细胞中调控胆碱摄取的关键作用，并发现其表达异常与神经系统疾病相关。
3. **文献名称**：*Recombinant CTL1 as a therapeutic target in cancer metabolism*
**作者**：Hosaka T, et al.
**摘要**：研究通过体外重组CTL1蛋白实验，揭示其在癌细胞中通过增强胆碱代谢促进脂质合成的机制，提示其作为癌症治疗靶点的潜力。
(注：以上为模拟文献，实际引用请通过PubMed/Google Scholar检索具体论文。)
CTL1 (Choline Transporter-Like Protein 1) is a transmembrane protein encoded by the *SLC44A1* gene, belonging to the solute carrier 44 (SLC44) family. It facilitates choline transport across cell membranes, a critical process for synthesizing phosphatidylcholine (a key phospholipid in cell membranes) and acetylcholine (a neurotransmitter). Unlike high-affinity choline transporters like CHT1. CTL1 operates with lower affinity but broader tissue distribution, functioning in the plasma membrane, mitochondria, and endoplasmic reticulum. Its activity supports cellular homeostasis, neurotransmission, and lipid metabolism.
Research highlights CTL1's role in diverse physiological contexts. In the nervous system, it regulates choline uptake for myelin synthesis and neuronal signaling. In non-neuronal tissues (e.g., epithelial cells, immune cells), it influences cell proliferation, inflammation, and mitochondrial function. Dysregulation of CTL1 has been linked to pathologies such as Alzheimer’s disease, cancer, and autoimmune disorders, where altered choline metabolism contributes to disease progression.
Recombinant CTL1 protein, produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), enables mechanistic studies of its transport kinetics, structure-function relationships, and interactions with pharmacological agents. Purified CTL1 is used to develop assays for screening modulators, investigate substrate specificity, and generate antibodies for diagnostic applications. Recent studies also explore its potential as a therapeutic target, particularly in cancers reliant on choline for membrane synthesis. Despite progress, questions remain about its regulatory mechanisms and tissue-specific isoforms, driving ongoing research to clarify its biological and clinical significance.
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