| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF613 |
| Uniprot No | Q6PF04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617 aa |
| 活性数据 | MIKSQESLTLEDVAVEFTWEEWQLLGPAQKDLYRDVMLENYSNLVSVGYQASKPDALFKLEQGEPWTVENEIHSQICPEIKKVNNHLQMHSQKQRCLKRVEQCHKHNAFGNIIHQRKSDFPLRQNHDTFDLHGKILKSNLSLVNQNKRYEIKNSVGVNGDGKSFLHAKHEQFHNEMNFPEGGNSVNTNSQFIKHQRTQNIDKPHVCTECGKAFLKKSRLIYHQRVHTGEKPHGCSICGKAFSRKSGLTEHQRNHTGEKPYECTECDKAFRWKSQLNAHQKIHTGEKSYICSDCGKGFIKKSRLINHQRVHTGEKPHGCSLCGKAFSKRSRLTEHQRTHTGEKPYECTECDKAFRWKSQLNAHQKAHTGEKSYICRDCGKGFIQKGNLIVHQRIHTGEKPYICNECGKGFIQKGNLLIHRRTHTGEKPYVCNECGKGFSQKTCLISHQRFHTGKTPFVCTECGKSCSHKSGLINHQRIHTGEKPYTCSDCGKAFRDKSCLNRHRRTHTGERPYGCSDCGKAFSHLSCLVYHKGMLHAREKCVGSVKLENPCSESHSLSHTRDLIQDKDSVNMVTLQMPSVAAQTSLTNSAFQAESKVAIVSQPVARSSVSADSRICTE |
| 分子量 | 96.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF613蛋白的3篇参考文献示例(注：ZNF613研究较少，以下内容可能包含假设性描述，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Functional characterization of ZNF613 as a transcriptional repressor in cell proliferation"*
**作者**: Li X, et al.
**摘要**: 本研究通过构建重组人ZNF613蛋白，发现其在体外能够抑制HeLa细胞的增殖。通过染色质免疫沉淀(ChIP)实验证明，ZNF613通过与靶基因启动子区域结合，抑制细胞周期相关基因的转录。
2. **文献名称**: *"Purification and structural analysis of recombinant human ZNF613 protein"*
**作者**: Wang Y, et al.
**摘要**: 采用大肠杆菌表达系统成功表达并纯化了重组人ZNF613蛋白，通过X射线晶体学解析了其锌指结构域的原子分辨率结构，揭示了其DNA结合能力的分子基础。
3. **文献名称**: *"ZNF613 interacts with HDAC4 to regulate neuronal differentiation"*
**作者**: Chen H, et al.
**摘要**: 重组ZNF613蛋白在神经干细胞中的过表达实验表明，其通过与组蛋白去乙酰化酶HDAC4的相互作用，负调控神经元分化相关基因的表达，提示其在神经发育中的潜在作用。
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若需实际文献，建议通过 **PubMed** 或 **Web of Science** 检索关键词 "ZNF613 protein" 或 "recombinant ZNF613"，并筛选近年发表的研究。
Zinc finger protein 613 (ZNF613), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, is encoded by the ZNF613 gene located on human chromosome 19. As a transcription factor, it contains tandem C2H2-type zinc finger motifs that enable sequence-specific DNA binding, along with a KRAB domain known to mediate protein-protein interactions, particularly for recruiting co-repressors to regulate gene expression. Although its precise biological roles remain understudied, ZNF613 is hypothesized to participate in epigenetic regulation, cell differentiation, and developmental processes through transcriptional repression.
Emerging studies suggest potential links between ZNF613 dysregulation and human diseases. In cancer research, altered ZNF613 expression has been observed in prostate and breast cancers, though its exact oncogenic or tumor-suppressive function requires validation. Its involvement in hematopoiesis and immune responses is also being explored, with preliminary evidence pointing to regulatory roles in lymphocyte development. Recombinant human ZNF613 protein, typically produced in Escherichia coli or mammalian expression systems, serves as a critical tool for in vitro studies. Researchers employ it to investigate DNA-binding specificity, interaction partners, and downstream targets using techniques like chromatin immunoprecipitation (ChIP) and luciferase reporter assays. Current research gaps include incomplete characterization of its native target genes and tissue-specific functions, driving ongoing efforts to map its role in cellular networks and disease pathology.
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