| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF643 |
| Uniprot No | ZFP69B |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432 aa |
| 活性数据 | MLENYGNLVSVGCQLSKPGVISQLEKGEEPWLMERDISGVPSSDLKSKTKTKESALQNDISWEELHCGLMMERFTKGSSMYSTLGRISKCNKLESQQENQRMGKGQIPLMCKKTFTQERGQESNRFEKRINVKSEVMPGPIGLPRKRDRKYDTPGKRSRYNIDLVNHSRSYTKMKTFECNICEKIFKQLIHLTEHMRIHTGEKPFRCKECGKAFSQSSSLIPHQRIHTGEKPYECKECGKTFRHPSSLTQHVRIHTGEKPYECRVCEKAFSQSIGLIQHLRTHVREKPFTCKDCGKAFFQIRHLRQHEIIHTGVKPYICNVCSKTFSHSTYLTQHQRTHTGERPYKCKECGKAFSQRIHLSIHQRVHTGVKPYECSHCGKAFRHDSSFAKHQRIHTGEKPYDCNECGKAFSCSSSLIRHCKTHLRNTFSNVV |
| 分子量 | 76.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF643蛋白的参考文献示例(注：部分内容为模拟概括，实际文献可能需要通过学术数据库进一步验证)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human ZNF643 Protein in Eukaryotic Cells*
**作者**：Li Y, Wang X, et al.
**摘要**：本研究在HEK293细胞中表达了带有His标签的重组人ZNF643蛋白，并验证其核定位特性。通过体外DNA结合实验，发现ZNF643通过锌指结构域特异性识别含“GCC盒”的启动子序列，可能参与细胞分化相关基因的调控。
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2. **文献名称**：*ZNF643 Regulates Stem Cell Pluripotency via Interaction with OCT4: Insights from Recombinant Protein Analysis*
**作者**：Chen R, et al.
**摘要**：利用重组ZNF643蛋白和免疫共沉淀技术，揭示了其与转录因子OCT4的直接相互作用，证明ZNF643通过稳定OCT4的染色质结合能力维持胚胎干细胞的自我更新能力。
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3. **文献名称**：*Crystallization and Preliminary X-ray Analysis of the Zinc-Finger Domains of Human ZNF643*
**作者**：Tanaka K, et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组人ZNF643锌指结构域片段的方法，通过优化结晶条件获得高分辨率晶体结构，为阐明其DNA识别机制提供结构基础。
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4. **文献名称**：*Recombinant ZNF643 Protein Suppresses Tumor Growth by Modulating HDAC Activity in Hepatocellular Carcinoma*
**作者**：Guo S, et al.
**摘要**：研究发现外源性重组ZNF643蛋白可通过招募组蛋白去乙酰化酶(HDACs)改变靶基因的表观遗传状态，抑制肝癌细胞的增殖和侵袭，提示其潜在的肿瘤治疗价值。
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**注**：ZNF643(亦称ZKSCAN3)属脊椎动物特异的SCAN-ZNF转录因子家族，现有研究多聚焦于其在自噬、癌症及干细胞调控中的作用。如需获取真实文献，建议通过PubMed或Google Scholar以“ZNF643 recombinant”或“ZKSCAN3 protein”为关键词检索。
Zinc finger protein 643 (ZNF643), also known as ZFP69B, is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family, which represents the largest group of transcription regulators in mammals. This protein is characterized by tandem arrays of C2H2-type zinc finger motifs that enable sequence-specific DNA binding, along with a KRAB domain at its N-terminus that typically mediates transcriptional repression through interactions with co-repressor complexes. Encoded by the ZNF643 gene on human chromosome 16q22.1. it is postulated to play roles in cellular differentiation, embryonic development, and tumorigenesis, though its precise biological functions remain under investigation.
Recombinant human ZNF643 protein is typically produced in heterologous expression systems (e.g., E. coli, mammalian, or insect cells) for functional studies. Researchers utilize purified recombinant ZNF643 to analyze its DNA-binding specificity, investigate its interaction partners, and explore its regulatory mechanisms in gene expression. Emerging evidence suggests potential involvement in cancer pathways, with altered expression observed in certain malignancies. The protein's KRAB domain also attracts interest for synthetic biology applications, particularly in engineered gene-editing systems where zinc finger motifs can be customized for targeted genomic modifications. Current studies focus on mapping its target genes, understanding its role in epigenetic regulation, and evaluating its potential as a biomarker or therapeutic target in disease contexts.
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