| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF625 |
| Uniprot No | Q96I27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306 aa |
| 活性数据 | MGERLLESKKDHQHGEILTQVPDDMLKKKTPRVKSCGEVSVGHASLNRHHRADTGHKPYEYQEYGQKPYKCTYCKKAFSDLPYFRTHEWAHTGGKPYDCEECGKSFISRSSIRRHRIMHSGDGPYKCNFCGKALMCLSLYLIHKRTHTGEKPYECKQCGKAFSHSGSLRIHERTHTGEKPYECSECGKAFHSSTCLHAHKITHTGEKPYECKQCGKAFVSFNSVRYHERTHTGEKPYECKQCGKAFRSASHLRTHGRTHTGEKPYECKQCGKAFGCASSVKIHERTHTGEKPCSSNTSKGQGEKIA |
| 分子量 | 33.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF625蛋白的参考文献示例(内容基于学术研究常见方向整理，部分信息可能为示例性概括)：
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1. **标题**: *Expression and Purification of Recombinant Human ZNF625 Protein in Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 该研究报道了通过大肠杆菌表达系统成功表达并纯化重组人ZNF625蛋白，优化了诱导条件和纯化步骤，为后续功能研究提供了高纯度蛋白来源。
2. **标题**: *Structural Insights into the DNA-Binding Properties of ZNF625 Zinc Finger Protein*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析ZNF625蛋白的锌指结构域，揭示了其与特定DNA序列结合的分子机制，表明其在基因转录调控中的潜在作用。
3. **标题**: *ZNF625 as a Novel Biomarker in Hepatocellular Carcinoma: Role in Tumor Progression*
**作者**: Wang Y, et al.
**摘要**: 研究证实ZNF625在肝癌组织中高表达，重组蛋白实验表明其通过调控细胞周期相关基因促进肿瘤增殖，提示其作为治疗靶点的潜力。
4. **标题**: *Functional Screening of ZNF625 Interaction Partners Using Yeast Two-Hybrid System*
**作者**: Kim H, et al.
**摘要**: 利用重组ZNF625蛋白筛选相互作用蛋白，发现其与多个染色质重塑复合物组分互作，暗示其在表观遗传调控中的潜在功能。
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**备注**：以上文献为示例性概括，具体研究请以实际发表文献为准。若需真实文献，建议通过PubMed或Web of Science以“ZNF625 recombinant protein”为关键词检索，并结合近年研究筛选。
Zinc finger protein 625 (ZNF625) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by tandem Cys2His2 zinc finger motifs that mediate sequence-specific DNA or RNA interactions. As a transcription factor, ZNF625 is hypothesized to regulate gene expression by binding to promoter regions, though its precise biological functions remain poorly understood. The human ZNF625 gene is located on chromosome 19q13.43 and encodes a protein with a conserved KRAB domain at the N-terminus, which may act as a transcriptional repressor by recruiting chromatin-modifying complexes, and multiple zinc finger repeats at the C-terminus for nucleic acid binding. Recombinant ZNF625 protein is typically produced using expression systems like Escherichia coli or mammalian cell lines, often fused with tags (e.g., His-tag) to facilitate purification and detection. Studies suggest its potential involvement in cellular differentiation, stress response, and carcinogenesis, with altered expression observed in certain cancers. However, functional studies are limited, emphasizing the need for further research to elucidate its molecular mechanisms, interaction partners, and roles in disease pathways. Recombinant ZNF625 serves as a critical tool for in vitro assays, structural studies, and screening for binding targets. Its evolutionary conservation across vertebrates underscores its biological significance, though detailed mechanistic insights await exploration.
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