| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF645 |
| Uniprot No | Q8N7E2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-425 aa |
| 活性数据 | MNKMPAGEQECEYNKEGKYYSKGVKLVRKKKKIPGYRWGDIKINIIGEKDDLPIHFCDKCDLPIKIYGRIIPCKHAFCYHCANLYDKVGYKVCPRCRYPVLRIEAHKRGSVFMCSIVQQCKRTYLSQKSLQAHIKRRHKRARKQVTSASLEKVRPHIAPPQTEISEIPKRLQDRDHLSYIPPEQHTMVSLPSVQHMLQEQHNQPHKDIQAPPPELSLSLPFPIQWETVSIFTRKHGNLTVDHIQNNSDSGAKKPTPPDYYPECQSQPAVSSPHHIIPQKQHYAPPPSPSSPVNHQMPYPPQDVVTPNSVRSQVPALTTTYDPSSGYIIVKVPPDMNSPPLRAPQSQNGNPSASEFASHHYNLNILPQFTENQETLSPQFTQTDAMDHRRWPAWKRLSPCPPTRSPPPSTLHGRSHHSHQRRHRRY |
| 分子量 | 46.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF645蛋白的参考文献示例(均为虚构，仅供参考格式)：
1. **文献名称**: "Functional Characterization of Recombinant ZNF645 in Gene Silencing Pathways"
**作者**: Smith J., et al.
**摘要**: 研究利用大肠杆菌表达系统成功制备重组ZNF645蛋白，并证明其通过结合特定DNA序列抑制靶基因转录，可能与表观遗传调控相关。
2. **文献名称**: "Recombinant ZNF645 Protein Interaction with HDAC Complexes in vitro"
**作者**: Zhang L., et al.
**摘要**: 通过体外 pull-down 实验发现重组ZNF645蛋白与组蛋白去乙酰化酶(HDACs)相互作用，提示其在染色质重塑中的潜在功能。
3. **文献名称**: "Crystal Structure Analysis of ZNF645 Zinc Finger Domains Expressed in Mammalian Cells"
**作者**: Tanaka K., et al.
**摘要**: 利用哺乳动物HEK293细胞表达纯化的重组ZNF645蛋白，通过X射线衍射解析其锌指结构域的三维结构，揭示DNA结合位点。
4. **文献名称**: "Overexpression of ZNF645 Recombinant Protein Triggers Cell Cycle Arrest in Hepatocellular Carcinoma"
**作者**: Wang Y., et al.
**摘要**: 研究表明在肝癌细胞中过表达重组ZNF645蛋白可诱导G1期阻滞，提示其可能作为肿瘤抑制因子发挥作用。
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**注意**：以上文献为模拟示例，实际研究需通过 **PubMed、Web of Science** 或 **Google Scholar** 检索关键词如 "recombinant ZNF645 protein" 或 "ZNF645 overexpression" 获取真实文献。
ZNF645 is a human zinc finger protein belonging to the Krüppel-associated box (KRAB) domain-containing subfamily of transcription factors. Encoded by the ZNF645 gene on chromosome 19q13.43. it features a conserved C2H2-type zinc finger motif, which facilitates DNA binding and protein interactions. While its precise biological role remains unclear, studies suggest involvement in transcriptional regulation, potentially repressing target genes by recruiting chromatin-modifying complexes. Notably, ZNF645 has been linked to eye development, with mutations associated with high myopia (severe nearsightedness), though molecular mechanisms are not fully defined.
Recombinant human ZNF645 protein is typically produced in Escherichia coli or mammalian expression systems, enabling functional studies. Purification via affinity tags (e.g., His-tag) allows investigation of its DNA-binding specificity, interaction partners (e.g., HCRP1), and regulatory pathways. Research focuses on elucidating its role in ocular tissue development, epigenetic silencing, and disease pathology. Structural analysis of the recombinant protein aids in mapping functional domains and designing mechanistic studies. As a potential therapeutic target for myopia, further characterization of ZNF645 could advance understanding of genetic eye disorders and zinc finger protein biology. Current challenges include identifying direct gene targets and tissue-specific regulatory networks.
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