| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF644 |
| Uniprot No | Q9H582 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-105 aa |
| 活性数据 | MLIRQNLALDCKQKKSRSRSGSKKKMLTLPHGADEVYILRCRFCGLVFRGPLSVQEDWIKHLQRHIVNANLPRTGAGMVEVTSLLKKPASITETSFSLLMAEAAS |
| 分子量 | 38.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF644蛋白的3条参考文献示例(内容基于学术常见方向合理虚构，供参考)：
---
1. **名称**: *Expression and Functional Analysis of Recombinant Human ZNF644 Protein*
**作者**: Zhang Y. et al. (2020)
**摘要**: 本研究成功克隆并表达了重组人ZNF644蛋白，采用大肠杆菌系统进行纯化。实验证实该蛋白能够特异性结合GC-rich DNA序列，并抑制靶基因的转录活性，提示其在基因调控中的作用。
---
2. **名称**: *ZNF644 Protein Interacts with Histone Deacetylases (HDACs) in Epigenetic Regulation*
**作者**: Li X. et al. (2018)
**摘要**: 通过重组ZNF644蛋白的体外互作实验，发现其与HDAC1/2形成复合物，调控组蛋白修饰和染色质重塑，可能与近视相关信号通路有关。
---
3. **名称**: *Structural Characterization of ZNF644 and Its Role in Tumor Suppression*
**作者**: Wang T. et al. (2022)
**摘要**: 利用重组ZNF644蛋白解析其锌指结构域，并揭示其通过调控Wnt/β-catenin通路抑制肿瘤细胞增殖的分子机制。
---
**备注**：实际研究中，ZNF644蛋白的功能探索(如近视、癌症)较多，但直接针对重组蛋白制备的文献较少，建议结合具体数据库(如PubMed)以关键词检索最新进展。
ZNF644 is a member of the zinc finger protein family, characterized by multiple C2H2-type zinc finger domains that facilitate sequence-specific DNA binding and protein interactions. Primarily localized in the nucleus, it functions as a transcriptional regulator, potentially modulating gene expression through chromatin remodeling or direct interaction with transcriptional machinery. While its full biological role remains under investigation, studies link ZNF644 to ocular development, particularly in regulating axial length and refractive errors, with mutations associated with high myopia (severe nearsightedness). It may also influence cell proliferation, apoptosis, and differentiation pathways.
Recombinant human ZNF644 protein is typically produced using expression systems like *E. coli* or mammalian cells, engineered to include affinity tags (e.g., His-tag) for purification. This recombinant form enables functional studies, including DNA-binding assays, interactome mapping, and mechanistic exploration of its role in development and disease. Its application extends to *in vitro* studies for probing molecular pathways, validating disease-associated variants, and screening therapeutic compounds targeting zinc finger proteins. Current research gaps include clarifying its target genes, regulatory networks, and tissue-specific roles beyond ocular biology. As a reagent, recombinant ZNF644 accelerates both basic research and potential translational applications in genetic eye disorders.
×
