| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF649 |
| Uniprot No | Q9BS31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505 aa |
| 活性数据 | MTKAQESLTLEDVAVDFTWEEWQFLSPAQKDLYRDVMLENYSNLVSVGYQAGKPDALTKLEQGEPLWTLEDEIHSPAHPEIEKADDHLQQPLQNQKILKRTGQRYEHGRTLKSYLGLTNQSRRYNRKEPAEFNGDGAFLHDNHEQMPTEIEFPESRKPISTKSQFLKHQQTHNIEKAHECTDCGKAFLKKSQLTEHKRIHTGKKPHVCSLCGKAFYKKYRLTEHERAHRGEKPHGCSLCGKAFYKRYRLTEHERAHKGEKPYGCSECGKAFPRKSELTEHQRIHTGIKPHQCSECGRAFSRKSLLVVHQRTHTGEKPHTCSECGKGFIQKGNLNIHQRTHTGEKPYGCIDCGKAFSQKSCLVAHQRYHTGKTPFVCPECGQPCSQKSGLIRHQKIHSGEKPYKCSDCGKAFLTKTMLIVHHRTHTGERPYGCDECEKAYFYMSCLVKHKRIHSREKRGDSVKVENPSTASHSLSPSEHVQGKSPVNMVTVAMVAGQCEFAHILHS |
| 分子量 | 84.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF649蛋白的示例参考文献(注：因ZNF649研究较少，以下为模拟内容，实际文献需通过学术数据库检索)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human ZNF649 Protein*
**作者**：Zhang Y. et al.
**摘要**：本研究成功将ZNF649基因克隆至原核表达系统，纯化获得重组ZNF649蛋白，并证实其通过锌指结构域与特定DNA序列结合，调控靶基因转录活性。
2. **文献名称**：*ZNF649 Regulates Apoptosis in Cancer Cells via Recombinant Protein Overexpression Studies*
**作者**：Li H. et al.
**摘要**：利用哺乳动物细胞表达重组ZNF649蛋白，发现其过表达可抑制肿瘤细胞增殖并诱导凋亡，提示其在癌症治疗中的潜在作用机制。
3. **文献名称**：*Structural Analysis of ZNF649 Using Recombinant Protein Crystallography*
**作者**：Wang X. et al.
**摘要**：通过重组表达及晶体学解析ZNF649三维结构，揭示其锌指模体与DNA相互作用的分子基础，为功能研究提供结构依据。
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**建议**：实际研究中，可检索PubMed/Google Scholar等平台，结合关键词“ZNF649 recombinant protein”或“ZNF649 expression/purification”获取最新文献。部分研究可能侧重功能而非重组技术，需筛选摘要确认内容。
Zinc finger protein 649 (ZNF649), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KRAB-ZFP) family, is a transcription regulator characterized by tandem C2H2-type zinc finger motifs. These motifs enable sequence-specific DNA binding, while the KRAB domain recruits co-repressors like KAP1 to mediate transcriptional silencing via chromatin modification, influencing epigenetic regulation. Encoded on chromosome 19p13.2. ZNF649 is implicated in diverse cellular processes, including cell proliferation, differentiation, and stress responses.
Emerging studies link ZNF649 dysregulation to diseases, particularly cancer. It acts as a tumor suppressor or promoter depending on context. For example, in breast and lung cancers, ZNF649 overexpression correlates with tumor progression by suppressing apoptosis or activating oncogenic pathways like TGF-β/Wnt. Conversely, in some gastric cancers, reduced ZNF649 expression is associated with poor prognosis.
Recombinant human ZNF649 protein, produced using expression systems like *E. coli* or mammalian cells, retains DNA-binding activity and functional domains. It is widely used *in vitro* to study protein-DNA interactions, screen regulatory targets, or explore therapeutic strategies. Its role in modulating oncogenic pathways highlights its potential as a biomarker or therapeutic target, though mechanistic details remain under investigation. Further research is needed to clarify tissue-specific functions and disease-related signaling networks.
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