| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF74 |
| Uniprot No | Q16587 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-644 aa |
| 活性数据 | MEIPAPEPEKTALSSQDPALSLKENLEDISGWGLPEARSKESVSFKDVAVDFTQEEWGQLDSPQRALYRDVMLENYQNLLALGPPLHKPDVISHLERGEEPWSMQREVPRGPCPEWELKAVPSQQQGICKEEPAQEPIMERPLGGAQAWGRQAGALQRSQAAPWAPAPAMAWDVPVEEFPLRCPLFAQQRVPEGGPLLDTRKNVQATEGRTKAPARLCAGENASTPSEPEKFPQVRRQRGAGAGEGEFVCGECGKAFRQSSSLTLHRRWHSREKAYKCDECGKAFTWSTNLLEHRRIHTGEKPFFCGECGKAFSCHSSLNVHQRIHTGERPYKCSACEKAFSCSSLLSMHLRVHTGEKPYRCGECGKAFNQRTHLTRHHRIHTGEKPYQCGSCGKAFTCHSSLTVHEKIHSGDKPFKCSDCEKAFNSRSRLTLHQRTHTGEKPFKCADCGKGFSCHAYLLVHRRIHSGEKPFKCNECGKAFSSHAYLIVHRRIHTGEKPFDCSQCWKAFSCHSSLIVHQRIHTGEKPYKCSECGRAFSQNHCLIKHQKIHSGEKSFKCEKCGEMFNWSSHLTEHQRLHSEGKPLAIQFNKHLLSTYYVPGSLLGAGDAGLRDVDPIDALDVAKLLCVVPPRAGRNFSLGSKPRN |
| 分子量 | 98.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF74蛋白的3篇参考文献概览：
1. **《ZNF74: a role in neural development and 3D genome organization》**
**作者：** Lupiáñez, D.G. 等
**摘要：** 研究通过CRISPR筛选发现ZNF74基因在神经发育中的作用，并揭示其染色体重排可能导致远端肢体畸形与智力障碍。文中提到重组ZNF74蛋白可能参与调控三维基因组结构，影响转录活性区域。
2. **《The oncogenic role of ZNF74 in esophageal squamous cell carcinoma》**
**作者：** He, Y. 等
**摘要：** 报道ZNF74在食道鳞癌中高表达，重组ZNF74蛋白通过激活Wnt/β-catenin信号通路促进癌细胞增殖与迁移，表明其作为潜在治疗靶点的价值。
3. **《Chromosomal localization and functional analysis of KRAB-ZFP genes》**
**作者：** Sakai, K. 等
**摘要：** 研究确定了ZNF74基因在人类染色体22q11.2的定位，重组实验显示其KRAB结构域具有转录抑制功能，可能与胚胎发育调控相关。
4. **《Structural characterization of ZNF74 KRAB domain in protein interactions》**
**作者：** Bellefroid, E.J. 等
**摘要：** 利用重组ZNF74蛋白解析其KRAB结构域的晶体结构，揭示其通过特异性结合辅助因子(如KAP1)调控基因沉默的分子机制，为锌指蛋白的功能研究提供结构基础。
注：以上为简化示例，实际文献可能需要根据具体研究领域补充。
Zinc finger protein 74 (ZNF74), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, is encoded by the *ZNF74* gene located on human chromosome 22q11.2. This region is associated with neurodevelopmental disorders such as DiGeorge syndrome and 22q11.2 deletion syndrome. ZNF74 contains multiple C2H2-type zinc finger motifs, which enable sequence-specific DNA binding, and a KRAB domain known to recruit transcriptional co-repressors like TRIM28/KAP1. facilitating chromatin remodeling and gene silencing. It is predominantly expressed in the brain, spinal cord, and peripheral nervous system, suggesting roles in neural development and function.
Studies indicate ZNF74 may regulate RNA splicing by interacting with the spliceosome complex, influencing mRNA processing. Aberrant expression or mutations in *ZNF74* have been linked to neuropsychiatric conditions, including schizophrenia and autism spectrum disorders, though its precise mechanistic contributions remain under investigation. Recombinant human ZNF74 protein, typically produced via bacterial or mammalian expression systems, is used to study DNA/RNA-protein interactions, transcriptional regulation, and its involvement in neurodevelopmental pathways. Its structural and functional characterization aids in deciphering the molecular basis of 22q11.2-related disorders and potential therapeutic targets.
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