| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSCAN5 |
| Uniprot No | Q9BUG6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496 aa |
| 活性数据 | MAANCTSSWSLGESCSRPGLELPRSMASSETQLGNHDVDPEISHVNFRMFSCPKESDPIQALRKLTELCHLWLRPDLHTKEQILDMLVMEQFMISMPQELQVLVMMNGVQSCKDLEDLLRNNRRPKKWSVVTFHGKEYIVQDSDIEMAEAPSSVRDDLKDVSSQRASSVNQMRPGEGQAHRELQILPRVPALSRRQGEDFLLHKSIDVTGDPKSLRPKQTLEKDLKENREENPGLTSPEPQLPKSPTDLVRAKEGKDPPKIASVENVDADTPSACVVEREASTHSGNRGDALNLSSPKRSKPDASSISQEEPQGEATPVGNRESPGQAGMNSIHSPGPASPVSHPDGQEAKALPPFACDVCEKRFTCNSKLVIHKRSHTGGRLFQCNLCGKRFMQLISLQFHQRTHTGERPYTCDVCQKQFTQKSYLKCHKRSHTGEKPFECKDCKKVFTYRGSLKEHQRIHSGEKPYKCSKCPRAFSRLKLLRRHQKTHPEATSQ |
| 分子量 | 80.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZSCAN5蛋白的3篇文献简要整理(注：文献标题与作者为模拟，实际需根据具体研究补充完善)：
1. **文献名称**：ZSCAN5 regulates embryonic stem cell pluripotency by recruiting OCT4 to target gene promoters
**作者**：Zhang Y et al. (2017)
**摘要**：本研究证实ZSCAN5作为转录因子，通过与OCT4蛋白相互作用调控胚胎干细胞的多能性。重组ZSCAN5蛋白的体外实验表明其能特异性结合DNA靶点，激活多能性相关基因表达。
2. **文献名称**：Crystal structure and functional analysis of human ZSCAN5 reveals zinc finger-dependent DNA recognition
**作者**：Kim H et al. (2020)
**摘要**：文章解析了重组人ZSCAN5蛋白的晶体结构，揭示其锌指结构域与DNA结合的关键氨基酸位点，并通过荧光偏振实验验证了其对特定DNA序列的亲和力，为ZSCAN5的基因调控机制提供结构基础。
3. **文献名称**：ZSCAN5 promotes cell proliferation in hepatocellular carcinoma via transcriptional activation of MYC
**作者**：Patel R et al. (2021)
**摘要**：研究发现重组ZSCAN5蛋白在肝癌细胞中过表达可激活MYC原癌基因，促进细胞增殖。ChIP-seq和报告基因实验证实ZSCAN5直接结合MYC启动子区，提示其在肿瘤发生中的潜在作用。
**注**：以上文献为示例，实际引用需根据具体研究方向检索PubMed/Google Scholar等数据库。建议结合研究关键词如“ZSCAN5 recombinant protein”或“ZSCAN5 transcriptional regulation”进一步筛选。
Zscan5. a member of the zinc finger and SCAN domain-containing protein family, is encoded by the ZSCAN5 gene in humans. Zinc finger proteins are critical for transcriptional regulation, DNA binding, and chromatin remodeling. The SCAN domain facilitates protein-protein interactions, enabling these proteins to form regulatory complexes. ZSCAN5. specifically, is implicated in maintaining telomere integrity and modulating cellular proliferation. Studies suggest its involvement in embryonic development, stem cell pluripotency, and carcinogenesis, with altered expression observed in cancers like hepatocellular carcinoma. Recombinant human ZSCAN5 protein, synthesized via heterologous expression systems (e.g., E. coli or mammalian cells), retains functional domains for biochemical studies. This engineered protein aids in elucidating ZSCAN5’s molecular interactions, DNA-binding specificity, and role in telomere dynamics. Its applications span in vitro assays, structural analyses, and drug screening for therapies targeting telomere-related disorders or cancers. Current research focuses on deciphering ZSCAN5’s dual roles in transcriptional activation/repression and its crosstalk with epigenetic modifiers. However, its precise mechanisms and physiological relevance in tissue-specific contexts remain under investigation, highlighting a need for further functional genomics studies.
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