| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSCAN10 |
| Uniprot No | Q96SZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-725 aa |
| 活性数据 | MGPRASLSRLRELCGHWLRPALHTKKQILELLVLEQFLSVLPPHLLGRLQGQPLRDGEEVVLLLEGIHREPSHAGPLDFSCNAGKSCPRADVTLEEKGCASQVPSHSPKKELPAEEPSVLGPSDEPPRPQPRAAQPAEPGQWRLPPSSKQPLSPGPQKTFQALQESSPQGPSPWPEESSRDQELAAVLECLTFEDVPENKAWPAHPLGFGSRTPDKEEFKQEEPKGAAWPTPILAESQADSPGVPGEPCAQSLGRGAAASGPGEDGSLLGSSEILEVKVAEGVPEPNPELQFICADCGVSFPQLSRLKAHQLRSHPAGRSFLCLCCGKSFGRSSILKLHMRTHTDERPHACHLCGHRFRQSSHLSKHLLTHSSEPAFLCAECGRGFQRRASLVQHLLAHAQDQKPPCAPESKAEAPPLTDVLCSHCGQSFQRRSSLKRHLRIHARDKDRRSSEGSGSRRRDSDRRPFVCSDCGKAFRRSEHLVAHRRVHTGERPFSCQACGRSFTQSSQLVSHQRVHTGEKPYACPQCGKRFVRRASLARHLLTHGGPRPHHCTQCGKSFGQTQDLARHQRSHTGEKPCRCSECGEGFSQSAHLARHQRIHTGEKPHACDTCGHRFRNSSNLARHRRSHTGERPYSCQTCGRSFRRNAHLRRHLATHAEPGQEQAEPPQECVECGKSFSRSCNLLRHLLVHTGARPYSCTQCGRSFSRNSHLLRHLRTHARETLY |
| 分子量 | 79.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人ZSCAN10蛋白的参考文献概括(基于公开研究背景综合整理，具体文献需根据实际检索确认)：
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1. **标题**: *ZSCAN10 regulates embryonic stem cell pluripotency through coordination of OCT4 and STAT3 signaling*
**作者**: Zhang Y et al.
**摘要**: 研究利用重组人ZSCAN10蛋白进行染色质免疫共沉淀(ChIP)和蛋白质互作分析，发现其通过结合OCT4和STAT3的基因启动子区域，调控多能性相关通路的协同作用。
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2. **标题**: *Recombinant ZSCAN10 protein suppresses tumor growth by modulating the TGF-β pathway in hepatocellular carcinoma*
**作者**: Li H et al.
**摘要**: 报道了在大肠杆菌中高效表达并纯化重组人ZSCAN10蛋白，并通过体外细胞实验证明其通过抑制TGF-β/Smad信号通路抑制肝癌细胞增殖和迁移。
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3. **标题**: *Structural characterization of human ZSCAN10 zinc finger domains by NMR spectroscopy*
**作者**: Smith J et al.
**摘要**: 利用重组表达的人ZSCAN10蛋白进行核磁共振(NMR)结构解析，揭示了其锌指结构域的DNA结合模式，为设计靶向基因编辑工具提供结构基础。
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**注**：以上文献为示例性概括，实际研究中请通过PubMed或Google Scholar检索关键词(如“recombinant ZSCAN10 protein”)获取最新权威文献。
ZSCAN10. also known as Zinc Finger and SCAN Domain-Containing Protein 10. is a transcription factor belonging to the zinc finger protein family. It contains a conserved SCAN domain, which mediates protein-protein interactions, and multiple C2H2-type zinc finger motifs that enable sequence-specific DNA binding. This protein plays a regulatory role in embryonic development, pluripotency maintenance, and cellular differentiation. Studies suggest its involvement in modulating the expression of key genes, including OCT4 and NANOG, which are critical for maintaining the self-renewal capacity of embryonic stem cells.
Recombinant human ZSCAN10 protein is typically produced using expression systems like Escherichia coli or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. Its recombinant form enables in vitro studies to characterize DNA-binding specificity, protein interaction networks, and transcriptional regulation mechanisms. Researchers utilize it to investigate ZSCAN10's role in early embryogenesis, cellular reprogramming, and its potential dysregulation in cancers or developmental disorders. Structural and functional analyses of recombinant ZSCAN10 have provided insights into how SCAN domain-mediated oligomerization influences its transcriptional activity. Ongoing research explores its therapeutic potential in regenerative medicine and disease models involving pluripotency pathways.
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