| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSCAN23 |
| Uniprot No | Q3MJ62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389 aa |
| 活性数据 | MAITLTLQTAEMQEGLLAVKVKEEEEEHSCGPESGLSRNNPHTREIFRRRFRQFCYQESPGPREALQRLQELCHQWLRPEMHTKEQILELLVLEQFLTILPEELQAWVRQHRPVSGEEAVTVLEDLERELDDPGEQVLSHAHEQEEFVKEKATPGAAQESSNDQFQTLEEQLGYNLREVCPVQEIDGKAGTWNVELAPKREISQEVKSLIQVLGKQNGNITQIPEYGDTCDREGRLEKQRVSSSVERPYICSECGKSFTQNSILIEHQRTHTGEKPYECDECGRAFSQRSGLFQHQRLHTGEKRYQCSVCGKAFSQNAGLFHHLRIHTGEKPYQCNQCNKSFSRRSVLIKHQRIHTGERPYECEECGKNFIYHCNLIQHRKVHPVAESS |
| 分子量 | 42.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZSCAN23蛋白的示例参考文献(内容为示例性综合，建议通过学术数据库验证具体文献)：
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1. **文献名称**: "Recombinant Expression and DNA-Binding Characterization of Human ZSCAN23 Protein"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌系统中表达了重组人ZSCAN23蛋白，并通过电泳迁移实验(EMSA)证明其特异性结合DNA启动子区域的保守基序，提示其在转录调控中的潜在作用。
2. **文献名称**: "Structural Insights into ZSCAN23 Zinc Finger Domains by X-ray Crystallography"
**作者**: Tanaka K, et al.
**摘要**: 文章解析了重组ZSCAN23蛋白的晶体结构，揭示了其锌指结构域的三维构象，并探讨了关键氨基酸残基在靶DNA识别中的功能，为设计基于ZSCAN23的基因编辑工具提供依据。
3. **文献名称**: "ZSCAN23 Regulates Pluripotency in Embryonic Stem Cells via Interaction with OCT4"
**作者**: Wang Y, et al.
**摘要**: 通过免疫共沉淀和体外Pull-down实验，研究者发现重组ZSCAN23蛋白与OCT4直接结合，并协同调控多能性基因的表达，暗示其在干细胞命运决定中的重要作用。
4. **文献名称**: "High-Throughput Screening of ZSCAN23 Inhibitors for Anti-Cancer Drug Development"
**作者**: Gupta R, et al.
**摘要**: 利用重组ZSCAN23蛋白建立体外筛选平台，鉴定出多个小分子化合物可抑制其转录激活活性，为靶向ZSCAN23过表达的癌症治疗提供先导化合物。
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注：以上参考文献为基于ZSCAN家族蛋白常见研究方向的示例，实际文献需通过PubMed、Google Scholar等平台以关键词“ZSCAN23 recombinant”或“ZSCAN23 function”检索确认。
ZSCAN23 is a human zinc finger protein belonging to the SCAN domain-containing family, which plays roles in transcriptional regulation and chromatin remodeling. It contains a conserved SCAN domain at its N-terminus, facilitating protein-protein interactions, and multiple C2H2-type zinc finger motifs at the C-terminus for sequence-specific DNA binding. As a transcription factor, ZSCAN23 is implicated in modulating gene expression networks, particularly during embryonic development, cellular differentiation, and immune responses. Its expression is tissue-specific, with higher activity observed in testis, brain, and immune cells, suggesting potential roles in germ cell development, neurological functions, or immune regulation.
Recombinant human ZSCAN23 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Purified versions enable investigations into DNA-binding specificity, interaction partners, and structural features. Emerging evidence links ZSCAN23 to cancer progression through dysregulation of oncogenic or tumor-suppressive pathways, though its precise molecular mechanisms remain poorly characterized. Current research focuses on elucidating its target genes, epigenetic regulation capabilities, and potential as a therapeutic target. The protein's conserved zinc finger architecture also makes it a model for studying DNA-protein recognition patterns in gene regulation.
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