| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZW10 |
| Uniprot No | O43264 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-779 aa |
| 活性数据 | MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERDSVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILKSLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEKTPMPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIRFESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK |
| 分子量 | 115.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人ZW10蛋白的相关文献摘要：
1. **文献名称**：*Role of ZW10 in maintaining dynamic kinetore-microtubule interaction stability*
**作者**：Kops, G.J., et al.
**摘要**：研究揭示了ZW10在有丝分裂中通过协调RZZ复合体(ROD-ZWILCH-ZW10)调控动粒与微管动态结合的功能，确保染色体正确分离和纺锤体检验点的激活。
2. **文献名称**：*The Zeste White 10 gene controls mitotic chromosome cohesion by modulating the activity of the cohesion establishment factor ESCO2*
**作者**：Williams, B.C., et al.
**摘要**：证明ZW10通过影响ESCO2调控姐妹染色单体黏连蛋白的组装，其缺失会导致染色体分离异常和基因组不稳定性，揭示了其与黏连蛋白复合体的关联。
3. **文献名称**：*Human ZW10 is a centromere/kinetochore component essential for mitotic checkpoint signaling*
**作者**：Chan, G.K., et al.
**摘要**：发现ZW10是动粒结构和纺锤体检验点的关键组分，通过RNA干扰实验表明其缺失导致染色体错误排列和检验点失效，首次在人类细胞中验证其功能保守性。
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**注**：以上内容为虚拟示例，实际文献需通过PubMed或Google Scholar检索关键词“ZW10”、“kinetochore”、“mitotic checkpoint”获取近年研究。
**Background of Recombinant Human ZW10 Protein**
The Zeste White 10 (ZW10) protein, first identified in *Drosophila* for its role in meiotic chromosome segregation, is a conserved component of the mitotic checkpoint machinery in eukaryotes. In humans, ZW10 is critical for ensuring accurate chromosome alignment and segregation during mitosis. It functions as part of the Rod-Zwilch-ZW10 (RZZ) complex, which localizes to kinetochores and regulates the recruitment of checkpoint proteins like Mad1/Mad2 to monitor spindle-microtubule attachment errors. By signaling incomplete or improper attachments, ZW10 helps delay anaphase onset until all chromosomes achieve bipolar attachment, thereby preventing aneuploidy.
ZW10 also plays roles in membrane trafficking, particularly in Golgi structure maintenance and vesicle transport, linking mitotic fidelity with cellular organelle organization. Dysregulation of ZW10 is associated with mitotic defects, chromosomal instability, and cancer progression.
Recombinant human ZW10 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains these functional properties and is widely used in studies exploring spindle assembly checkpoint mechanisms, intracellular trafficking, and disease models. Its application extends to *in vitro* binding assays, structural studies, and screening for therapeutic agents targeting mitotic errors or trafficking disorders.
This protein remains a key focus in understanding cellular division fidelity and its implications in development and disease.
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