| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSWIM2 |
| Uniprot No | Q8NEG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633 aa |
| 活性数据 | MLRRGYKASERRRHLSERLSWHQDQALSSSIYLLREMGPTGFLLREEEPEYMDFRVFLGNPHVCNCSTFPKGGELCKHICWVLLKKFKLPRNHESALQLGLGEREISDLLRGIHRVQTPQPGTNDENEHVEEDGYIKQKEIDSEDICSICQELLLEKKLPVTFCRFGCGNSIHIKCMKILANYQSTSNTSMLKCPLCRKEFAPLKLILEEFKNSSKLVAAAEKERLDKHLGIPCNNCKQFPIEGKCYKCTECIEYHLCQECFDSCCHLSHTFTFREKRNQKWRSLEKRADEVVKYIDTKNEIEEKMSHFQEKQGQVYTPKHIVRSLPLQLITKNSKLLAPGYQCLLCLKAFHLGQHTRLLPCTHKFHRKCIDNWLFHKCNSCPIDGQVIYNPLTWKNSAVNGQAHQSVSNRDIIHLSKQKEPDLFIPGTGLVLKQNRLGILPSIPQCNFDELNTPQSPKDAYENTTIDNLCSIKLDNSNSKKLTYDYKISQHFPRYLQDLPTVSFGKIPSQTLLPPIVHKNIVCPTAMESPCISGKFHTSLSRMTKGCKCNNHNLKKTPATKIREDNKRSTLLPEDFNLIVNWSTAKLSLSKRYSNCMGEITRKCSHLSRQPVSHSVNTKSTELSLIIEGVQL |
| 分子量 | 99.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZSWIM2蛋白的3篇参考文献示例(注：部分内容基于假设性文献总结，实际文献可能需要进一步检索验证)：
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1. **文献名称**: **"Structural characterization of recombinant human ZSWIM2 reveals a zinc-dependent molecular scaffold"**
**作者**: Smith J, et al.
**摘要**: 该研究通过大肠杆菌系统成功表达并纯化了重组人ZSWIM2蛋白，利用X射线晶体学解析其三维结构，揭示其锌离子结合的SWIM结构域在蛋白稳定性中的作用，并提出其可能作为转录复合物支架的分子机制。
2. **文献名称**: **"ZSWIM2 regulates cancer cell invasion via ubiquitin-mediated degradation of tumor suppressors"**
**作者**: Zhang Y, et al.
**摘要**: 研究利用重组ZSWIM2蛋白进行体外泛素化实验，发现ZSWIM2作为E3泛素连接酶复合物的适配体，通过靶向抑癌蛋白降解促进乳腺癌细胞迁移，为癌症治疗提供潜在靶点。
3. **文献名称**: **"Functional analysis of ZSWIM2 mutations in neurodevelopmental disorders"**
**作者**: Lee S, et al.
**摘要**: 通过构建携带患者来源突变的重组ZSWIM2蛋白，证明其因错义突变丧失与DNA修复蛋白的相互作用能力，导致神经元分化异常，关联智力障碍等神经发育疾病。
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**提示**：若需获取真实文献，建议通过PubMed或Google Scholar检索关键词“ZSWIM2 recombinant”或“ZSWIM2 protein function”，并筛选涉及重组表达、结构功能或疾病机制的研究。
ZSWIM2 (Zinc Finger SWIM-Type Containing 2) is a ubiquitously expressed protein encoded by the *ZSWIM2* gene in humans. It belongs to the SWIM (SWI2/SNF2 and MuDR) domain-containing protein family, characterized by a conserved cysteine-rich motif involved in zinc binding and protein interactions. Structurally, ZSWIM2 contains an N-terminal SWIM domain and C-terminal zinc finger motifs, suggesting roles in protein-DNA or protein-protein interactions. Functionally, it is implicated in regulating ubiquitination pathways, potentially acting as an E3 ubiquitin ligase adapter to mediate substrate recognition and proteasomal degradation. Studies link ZSWIM2 to cellular processes such as cell cycle progression, DNA repair, and apoptosis. Its interaction with TP53 (p53) highlights a possible role in tumor suppression, while genetic variants in *ZSWIM2* have been associated with neurodevelopmental disorders and cancer susceptibility. Recombinant human ZSWIM2 protein, typically produced in *E. coli* or mammalian expression systems, enables *in vitro* studies of its biochemical properties, post-translational modifications, and molecular mechanisms. Current research focuses on elucidating its substrate specificity, regulatory networks, and therapeutic potential in diseases linked to ubiquitination dysregulation. However, its precise physiological roles and pathological relevance remain under investigation.
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