| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSUN2 |
| Uniprot No | Q08J23 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767aa |
| 活性数据 | MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR |
| 分子量 | 93.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人NSUN2蛋白的参考文献及其摘要概括(注：文献信息为模拟，供参考)：
1. **"Human NSUN2 methylates tRNA and promotes tumor progression through translational regulation"**
- **作者**: Yang et al.
- **摘要**: 研究揭示了重组人NSUN2蛋白通过催化tRNA的m5C甲基化修饰，增强致癌相关mRNA的翻译效率，从而促进肿瘤细胞的增殖和转移。
2. **"Crystal structure of human NSUN2 reveals conformational changes during RNA binding"**
- **作者**: Martinez et al.
- **摘要**: 利用重组NSUN2蛋白解析了其晶体结构，阐明了RNA底物结合时酶活位点的构象变化，为靶向NSUN2的药物设计提供结构基础。
3. **"Recombinant NSUN2 regulates DNA damage response via rRNA modification"**
- **作者**: Squires et al.
- **摘要**: 发现重组NSUN2蛋白通过核糖体RNA的甲基化修饰，调控DNA损伤应答通路，NSUN2缺失导致细胞对基因组不稳定性敏感。
建议通过PubMed或SciHub检索关键词 **"recombinant NSUN2"** 或 **"NSUN2 methyltransferase"** 获取真实文献。
NSUN2 (NOP2/Sun RNA methyltransferase 2) is a conserved RNA methyltransferase in eukaryotes that catalyzes the transfer of methyl groups to cytosine residues in RNA, forming 5-methylcytosine (m5C) modifications. This enzyme belongs to the NSUN protein family and plays a critical role in regulating RNA stability, translation, and cellular stress responses. Structurally, NSUN2 contains a catalytic domain with conserved methyltransferase motifs and interacts with various RNA substrates, including tRNAs, mRNAs, and non-coding RNAs, influencing their functional dynamics. Its activity is essential for normal cellular processes such as proliferation, differentiation, and DNA damage repair. Dysregulation of NSUN2 has been implicated in developmental disorders, neurological diseases, and cancers, underscoring its biological and clinical significance. Recombinant human NSUN2 protein is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and enzymatic activity. This recombinant tool enables in vitro studies on m5C modification mechanisms, substrate specificity, and interactions with cofactors. It also serves as a key reagent for screening inhibitors or modulators targeting NSUN2-related pathologies. Current research leverages recombinant NSUN2 to explore RNA epigenetics and its potential as a therapeutic target, particularly in cancer and neurodegeneration. Its study advances understanding of post-transcriptional gene regulation and disease-associated RNA modifications.
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