| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZZZ3 |
| Uniprot No | Q8IYH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409 aa |
| 活性数据 | MIDLWLYSYQRLLQTIAVLEAQRSQAVQDLESLGRHQREALKNPIGFVEKLQKKADIGLPYPQRVVQLPEIVWDQYTHSLGNFEREFKNRKRHTRRVKLVFGKVGLPARPKSPLDPKKDGESLSYSMLPLSDGPEGSSSRPQMIRGRLCDDTKPETFNQLWTVEEQKKLEQLLIKYPPEEVESRRWQKIADELGNRTAKQVASQVQKYFIKLTKAGIPVPGRTPNLCIYSKKSSTSRRQHPLNKHLFKPSTFMTSHEPPVYMDEDDDRSCFHSHMNTAVEDASDDESIPIMYRNLPEYKELLQFKKLKKQKLQQMQAESGFVQHVGFKCDNCGIEPIQGVRWHCQDCPPEMSLDFCDSCSDCLHETDIHKEDHQLEPIYRSETFLDRDYCVSQGTSYNYLDPNYFPANR |
| 分子量 | 74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人ZZZ3蛋白的虚构参考文献示例(注：ZZZ3蛋白名称或功能为假设，实际文献需替换为真实来源)：
1. **文献名称**: *Structural and Functional Analysis of Recombinant Human ZZZ3 Protein*
**作者**: Zhang L, Wang X, Chen R.
**摘要**: 本研究在大肠杆菌中表达了重组人ZZZ3蛋白，并解析了其晶体结构。发现ZZZ3通过N端结构域与DNA结合，可能在表观遗传调控中参与组蛋白修饰复合体的组装。
2. **文献名称**: *Recombinant ZZZ3 Promotes Neural Stem Cell Differentiation via Wnt Signaling*
**作者**: Tanaka K, et al.
**摘要**: 利用HEK293细胞系统生产功能性重组ZZZ3蛋白，发现其通过激活Wnt/β-catenin通路促进小鼠神经干细胞的神经元分化，提示其在神经再生治疗中的潜在应用。
3. **文献名称**: *Proteomic Identification of ZZZ3-Interacting Partners in Cancer Cells*
**作者**: Gupta S, Lee TM, Kim H.
**摘要**: 通过亲和纯化-质谱技术，鉴定出重组人ZZZ3蛋白与多个肿瘤抑制蛋白(如p53、BRCA1)存在相互作用，可能通过调控基因组稳定性影响癌细胞增殖。
4. **文献名称**: *Development of a High-Yield ZZZ3 Protein Production System in Pichia pastoris*
**作者**: Müller J, et al.
**摘要**: 优化毕赤酵母表达系统实现重组人ZZZ3蛋白的高效分泌表达(产量达120 mg/L)，糖基化修饰显著增强了其体外酶活性稳定性。
注：以上为模拟生成内容，实际研究中建议通过PubMed/Google Scholar检索真实文献(关键词：Recombinant human ZZZ3 protein)。若需真实文献分析，请提供更具体的蛋白功能描述。
Recombinant human ZZZ3 protein is a genetically engineered biomolecule produced through heterologous expression systems, such as *E. coli*, yeast, or mammalian cells. ZZZ3. a hypothetical or less-characterized protein (name substituted for illustration), is postulated to play roles in cellular processes like transcriptional regulation, signal transduction, or post-translational modifications based on sequence homology or preliminary studies. Its recombinant form enables controlled production for functional and structural analyses, overcoming challenges in isolating it from native tissues due to low abundance or instability.
The protein typically includes affinity tags (e.g., His-tag, GST) to facilitate purification and detection. Structural studies may reveal domains indicative of enzymatic activity (kinase, protease) or binding motifs (SH3. zinc finger), suggesting interactions with nucleic acids, proteins, or small molecules. Recombinant ZZZ3 is pivotal for *in vitro* assays (e.g., kinase activity, protein-protein interaction) and *in vivo* models to elucidate its biological significance in pathways linked to diseases like cancer, neurodegeneration, or immune disorders.
Biopharmaceutical interest in ZZZ3 stems from its potential as a therapeutic target or diagnostic marker. Expression optimization (codon usage, host selection) ensures proper folding and post-translational modifications (phosphorylation, glycosylation) critical for functional studies. Current research focuses on validating its interactome, resolving 3D structures, and evaluating its role in disease mechanisms using knockout models or inhibitor screens. Further characterization may bridge gaps in understanding its physiological and pathological relevance.
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