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Recombinant  Human ZYG11B Protein

  • 中文名: 重组人(ZYG11B)蛋白
  • 别    名: Protein zyg-11 homolog B
货号: PAX2000-13047
Price: ¥询价
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纯度>90%SDS-PAGE.
种属Human
靶点ZYG11B
Uniprot NoQ9C0D3
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-390 aa
活性数据MEKTKPEILKLVVTGMRNHPMNLPVQLAASACVFNLTKQDLAAGMPVRLLADVTHLLLKAMEHFPNHQQLQKNCLLSLCSDRILQDVPFNRFEAAKLVMQWLCNHEDQNMQRMAVAIISILAAKLSTEQTAQLGTELFIVRQLLQIVKQKTNQNSVDTTLKFTLSALWNLTDESPTTCRHFIENQGLELFMRVLESFPTESSIQQKVLGLLNNIAEVQELHSELMWKDFIDHISSLLHSVEVEVSYFAAGIIAHLISRGEQAWTLSRSQRNSLLDDLHSAILKWPTPECEMVAYRSFNPFFPLLGCFTTPGVQLWAVWAMQHVCSKNPSRYCSMLIEEGGLQHLYNIKDHEHTDPHVQQIAVAILDSLEKHIVRHGRPPPCKKQPQARLN
分子量68.64 kDa
蛋白标签GST-tag at N-terminal
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人ZYG11B蛋白的3篇模拟参考文献示例(注:文献为虚构示例,仅供参考格式):

1. **"ZYG11B interacts with APC/C complex to regulate cell cycle progression via ubiquitination"**

*Zhang, L. et al. (2020). Journal of Biological Chemistry.*

摘要:研究揭示了ZYG11B作为APC/C-E3泛素连接酶复合体的适配蛋白,通过促进特定细胞周期蛋白的泛素化降解调控G1/S期转换。重组ZYG11B蛋白实验证实其与APC/C的相互作用依赖保守结构域。

2. **"Downregulation of ZYG11B inhibits colorectal cancer proliferation by inducing apoptosis"**

*Li, X. et al. (2019). Cancer Research.*

摘要:通过重组ZYG11B蛋白敲低模型,发现其缺失导致结直肠癌细胞周期阻滞并激活caspase通路,提示ZYG11B通过抑制凋亡促进肿瘤生长,可能成为潜在治疗靶点。

3. **"Structural basis of ZYG11B phosphorylation in DNA damage response"**

*Chen, Y. et al. (2021). Cell Reports.*

摘要:利用重组ZYG11B蛋白的晶体结构分析,揭示其Ser-215位点磷酸化在DNA损伤修复中的关键作用,磷酸化修饰增强其与BRCA1的互作,维持基因组稳定性。

注:实际研究中请通过PubMed、Google Scholar等平台检索真实文献。


背景信息

ZYG11B (Zyg-11 Family Member B) is a human protein encoded by the ZYG11B gene, belonging to the Zyg-11 family known for regulating cell cycle progression and protein degradation. Structurally, it contains tandem tetratricopeptide repeat (TPR) domains, facilitating protein-protein interactions. Research links ZYG11B to the ubiquitin-proteasome system, where it may act as a substrate receptor for E3 ubiquitin ligase complexes, targeting proteins for proteasomal degradation.

This protein is implicated in mitotic regulation and chromosomal stability. Studies highlight its overexpression in cancers, including colorectal and breast cancer, suggesting a role in tumorigenesis by disrupting cell cycle checkpoints. Conversely, reduced ZYG11B expression correlates with neurodegenerative diseases, emphasizing its tissue-specific functions. Recent work also explores its involvement in autophagy and mitochondrial dynamics.

Recombinant ZYG11B protein is typically produced in *E. coli* or mammalian expression systems for functional studies. Its purified form enables biochemical assays (e.g., ubiquitination assays), structural studies, and antibody development. Researchers utilize it to map interaction networks, decode signaling pathways, and evaluate therapeutic targeting potential. Despite progress, ZYG11B's full mechanistic spectrum remains under investigation, particularly its dual roles in cancer suppression/progression and organelle homeostasis. This recombinant tool accelerates efforts to define its physiological and pathological relevance.


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