| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZXDA |
| Uniprot No | P98168 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-799 aa |
| 活性数据 | MEIPKLLPARGTLQGGGGGGIPAGGGRVHRGPDSPAGQVPTRRLLLPRGPQDGGPGRRREEASTASRGPGPSLFAPRPHQPSGGGDDFFLVLLDPVGGDVETAGSGQAAGPVLREEAKAGPGLQGDESGANPAGCSAQGPHCLSAVPTPAPISAPGPAAAFAGTVTIHNQDLLLRFENGVLTLATPPPHAWEPGAAPAQQPRCLIAPQAGFPQAAHPGDCPELRSDLLLAEPAEPAPAPAPQEEAEGLAAALGPRGLLGSGPGVVLYLCPEALCGQTFAKKHQLKMHLLTHSSSQGQRPFKCPLGGCGWTFTTSYKLKRHLQSHDKLRPFGCPAEGCGKSFTTVYNLKAHMKGHEQENSFKCEVCEESFPTQAKLGAHQRNHFEPERPYQCAFSGCKKTFITVSALFSHNRAHFREQELFSCSFPGCSKQYDKACRLKIHLRSHTGERPFLCDFDGCGWNFTSMSKLLRHKRKHDDDRRFMCPVEGCGKSFTRAEHLKGHSITHLGTKPFVCPVAGCCARFSARSSLYIHSKKHLQDVDTWKSRCPISSCNKLFTSKHSMKTHMVKRHKVGQDLLAQLEAANSLTPSSELTSQRQNDLSDAEIVSLFSDVPDSTSAALLDTALVNSGILTIDVASVSSTLAGHLPANNNNSVGQAVDPPSLMATSDPPQSLDTSLFFGTAATGFQQSSLNMDEVSSVSVGPLGSLDSLAMKNSSPEPQALTPSSKLTVDTDTLTPSSTLCENSVSELLTPAKAEWSVHPNSDFFGQEGETQFGFPNAAGNHGSQKERNLITVTGSSFLV |
| 分子量 | 87.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为虚构的关于重组人ZXDA蛋白的参考文献示例(ZXDA为假设蛋白名，仅用于演示格式)：
1. **文献名称**: "Expression and purification of recombinant human ZXDA in E. coli for structural studies"
**作者**: Smith J.R., Chen L.
**摘要**: 本研究描述了一种利用大肠杆菌表达系统高效表达可溶性ZXDA蛋白的方法，并通过镍柱亲和层析和凝胶过滤技术纯化，获得高纯度蛋白用于X射线晶体学分析。
2. **文献名称**: "Crystal structure of ZXDA reveals a novel zinc-binding domain critical for enzymatic activity"
**作者**: Tanaka M., Wang Y.
**摘要**: 通过解析ZXDA蛋白的2.1Å晶体结构，发现其C端存在新型锌结合模体，体外酶活实验证实该区域对其催化功能至关重要。
3. **文献名称**: "Functional characterization of ZXDA in regulating cell cycle progression"
**作者**: Gupta S. et al.
**摘要**: 研究发现重组ZXDA蛋白通过磷酸化p53蛋白调控G1/S期转换，过表达ZXDA可抑制多种癌细胞增殖，提示其作为肿瘤治疗靶点的潜力。
4. **文献名称**: "Development of a ZXDA-specific monoclonal antibody using recombinant protein immunization"
**作者**: Lee H.K., Park S.
**摘要**: 利用重组ZXDA蛋白免疫小鼠获得高特异性单克隆抗体，经ELISA和免疫印迹验证其灵敏度和交叉反应性，为ZXDA的病理检测提供工具。
---
**注**：上述文献为虚构示例，ZXDA蛋白不存在于现有数据库中。建议通过PubMed或Google Scholar检索真实文献，并检查蛋白名称拼写(如是否为ZXA、ZXD1等类似名称)。如需特定领域文献，请提供更多背景信息。
Recombinant human ZXDA protein is a genetically engineered version of the zinc finger X-linked duplicated protein A (ZXDA), a transcription factor encoded by the ZXDA gene located on the X chromosome. ZXDA belongs to the zinc finger protein family, characterized by conserved cysteine-rich domains that mediate DNA, RNA, or protein interactions. While the exact biological role of ZXDA remains under investigation, zinc finger proteins are generally involved in gene regulation, cellular differentiation, and developmental processes.
The recombinant form is produced using expression systems like *E. coli* or mammalian cells, enabling high-purity yields for functional studies. Its production often includes fusion tags (e.g., His-tag) to facilitate purification and detection. Research on recombinant ZXDA focuses on elucidating its molecular interactions, DNA-binding specificity, and potential roles in pathways linked to genetic disorders or cancer. Due to zinc finger proteins’ modular structure, ZXDA has also garnered interest in synthetic biology for designing customized DNA-binding tools. Current applications span structural studies, protein interaction assays, and screening for therapeutic compounds targeting transcriptional dysregulation. Limited clinical data exist, but its study may contribute to understanding X-linked diseases or zinc finger-related pathologies.
×
