**Background of PTPRQ Recombinant Protein**
PTPRQ (Protein Tyrosine Phosphatase Receptor Type Q) is a transmembrane protein belonging to the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues. Structurally, PTPRQ is characterized by a large extracellular domain, a single transmembrane region, and an intracellular catalytic domain with phosphatase activity. It is predominantly expressed in sensory tissues, including the inner ear, where it is essential for maintaining auditory function. Studies suggest that PTPRQ is involved in the development and homeostasis of stereocilia in hair cells, crucial for mechanoelectrical transduction in hearing.
Mutations in the *PTPRQ* gene are linked to autosomal recessive deafness in humans, highlighting its importance in auditory physiology. Recombinant PTPRQ protein, produced via heterologous expression systems (e.g., mammalian or insect cells), enables detailed biochemical and functional studies. Researchers use it to investigate its enzymatic activity, interactions with cytoskeletal components (e.g., actin-binding proteins), and role in signaling pathways influencing cell adhesion and structural integrity.
Beyond basic research, recombinant PTPRQ holds therapeutic potential. It may serve as a tool for high-throughput drug screening to identify modulators of phosphatase activity or as a protein replacement strategy for hearing disorders caused by *PTPRQ* deficiencies. Additionally, its extracellular domain is being explored for diagnostic applications, such as detecting autoantibodies in autoimmune hearing loss.
Overall, PTPRQ recombinant protein is a vital resource for unraveling molecular mechanisms in auditory biology and developing targeted interventions for hearing-related pathologies.
以下是关于PTPRO重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*PTPRO suppresses tumor cell migration and invasion via regulation of STAT3 signaling in hepatocellular carcinoma*
**作者**：Chen L, et al.
**摘要**：该研究通过重组PTPRO蛋白体外实验，发现其过表达可抑制肝癌细胞迁移和侵袭，并诱导凋亡，机制与抑制STAT3磷酸化及下游靶基因调控相关。
2. **文献名称**：*Structural characterization of the extracellular domain of PTPRO reveals its role in cell-cell adhesion*
**作者**：Wang Y, et al.
**摘要**：利用重组PTPRO胞外域蛋白进行结构解析，发现其通过特定免疫球蛋白样结构域介导细胞间黏附，为PTPRO在肿瘤微环境中的作用提供结构依据。
3. **文献名称**：*Recombinant PTPRO attenuates oncogenic signaling by dephosphorylating mutant FLT3 in acute myeloid leukemia*
**作者**：Meng TC, et al.
**摘要**：研究表明重组PTPRO蛋白可特异性去磷酸化白血病中突变的FLT3受体，抑制异常增殖信号，提示其作为潜在治疗靶点的价值。
(注：上述文献信息为示例性内容，实际引用需以真实出版物为准。)
**Background of PTPRO Recombinant Protein**
PTPRO (Protein Tyrosine Phosphatase Receptor Type O), a member of the protein tyrosine phosphatase (PTP) superfamily, is a transmembrane receptor-like enzyme critical for regulating tyrosine phosphorylation-dependent signaling pathways. PTPRO plays a pivotal role in cellular processes such as cell growth, differentiation, apoptosis, and immune response modulation. Structurally, it contains an extracellular domain, a single transmembrane region, and a conserved intracellular catalytic domain responsible for dephosphorylating target proteins.
PTPRO is expressed in various tissues, including the kidney, brain, and hematopoietic cells, and has been implicated in both physiological and pathological contexts. Studies highlight its tumor-suppressive functions in cancers (e.g., leukemia, breast cancer, and hepatocellular carcinoma), where it often undergoes epigenetic silencing or downregulation. Conversely, PTPRO’s aberrant expression is linked to neurodegenerative disorders and autoimmune diseases, underscoring its context-dependent roles.
Recombinant PTPRO protein is engineered using biotechnological platforms (e.g., mammalian or bacterial expression systems) to produce purified, functional protein for research and therapeutic applications. This protein retains enzymatic activity, enabling studies on substrate interactions, signaling mechanisms, and inhibitor screening. Its recombinant form is vital for elucidating PTPRO’s structure-function relationships and developing targeted therapies.
Emerging interest in PTPRO stems from its potential as a biomarker and therapeutic target. Inhibitors or activators of PTPRO could modulate disease-related pathways, offering avenues for treating cancer, inflammation, or neurodegeneration. However, challenges remain in achieving specificity, as PTPs share structural homology. Overall, PTPRO recombinant protein serves as a key tool in decoding its biological significance and translational potential.
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