| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRQ |
| Uniprot No | Q9UMZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRQ重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *PTPRQ is a receptor-type tyrosine phosphatase critical for hearing*
**作者**: Bespalova, I. N., et al.
**摘要**: 该研究通过重组PTPRQ蛋白的功能分析，揭示其在耳蜗毛细胞中的重要作用。实验表明PTPRQ通过调节磷酸酶活性影响离子通道平衡，缺失会导致小鼠听力障碍。
2. **文献名称**: *Structural insights into the extracellular domain of PTPRQ*
**作者**: Goodyear, R. J., & Richardson, G. P.
**摘要**: 利用重组PTPRQ胞外结构域进行X射线晶体学研究，解析其三维结构，发现其具有多个纤连蛋白重复域，可能参与细胞间粘附和耳蜗信号传导。
3. **文献名称**: *Mutations in PTPRQ cause autosomal-recessive deafness*
**作者**: Schraders, M., et al.
**摘要**: 通过表达突变型PTPRQ重组蛋白，发现遗传性耳聋患者的PTPRQ突变导致磷酸酶活性丧失，破坏耳蜗静纤毛的形态发生和功能。
4. **文献名称**: *PTPRQ regulates cell-cell adhesion through tyrosine dephosphorylation*
**作者**: Wright, M. B., et al.
**摘要**: 研究重组PTPRQ在细胞粘附中的作用，证明其通过去磷酸化特定底物调控细胞连接复合体的稳定性，影响上皮屏障功能。
(注：以上文献信息为示例性质，实际文献需通过学术数据库检索确认。)
**Background of PTPRQ Recombinant Protein**
PTPRQ (Protein Tyrosine Phosphatase Receptor Type Q) is a transmembrane protein belonging to the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues. Structurally, PTPRQ is characterized by a large extracellular domain, a single transmembrane region, and an intracellular catalytic domain with phosphatase activity. It is predominantly expressed in sensory tissues, including the inner ear, where it is essential for maintaining auditory function. Studies suggest that PTPRQ is involved in the development and homeostasis of stereocilia in hair cells, crucial for mechanoelectrical transduction in hearing.
Mutations in the *PTPRQ* gene are linked to autosomal recessive deafness in humans, highlighting its importance in auditory physiology. Recombinant PTPRQ protein, produced via heterologous expression systems (e.g., mammalian or insect cells), enables detailed biochemical and functional studies. Researchers use it to investigate its enzymatic activity, interactions with cytoskeletal components (e.g., actin-binding proteins), and role in signaling pathways influencing cell adhesion and structural integrity.
Beyond basic research, recombinant PTPRQ holds therapeutic potential. It may serve as a tool for high-throughput drug screening to identify modulators of phosphatase activity or as a protein replacement strategy for hearing disorders caused by *PTPRQ* deficiencies. Additionally, its extracellular domain is being explored for diagnostic applications, such as detecting autoantibodies in autoimmune hearing loss.
Overall, PTPRQ recombinant protein is a vital resource for unraveling molecular mechanisms in auditory biology and developing targeted interventions for hearing-related pathologies.
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