| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLUNC |
| Uniprot No | Q9NP55 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-256aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSQFGGLPV PLDQTLPLNV NPALPLSPTG LAGSLTNALS NGLLSGGLLG ILENLPLLDI LKPGGGTSGG LLGGLLGKVT SVIPGLNNII DIKVTDPQLL ELGLVQSPDG HRLYVTIPLG IKLQVNTPLV GASLLRLAVK LDITAEILAV RDKQERIHLV LGDCTHSPGS LQISLLDGLG PLPIQGLLDS LTGILNKVLP ELVQGNVCPL VNEVLRGLDI TLVHDIVNML IHGLQFVIKV |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLUNC重组蛋白的参考文献及其简要摘要：
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1. **标题**: *Recombinant human PLUNC protein has antibacterial activity against respiratory pathogens*
**作者**: Bingle, C.D., et al.
**摘要**: 研究报道了重组人PLUNC蛋白在体外对铜绿假单胞菌和流感嗜血杆菌的抑菌活性，揭示了其在先天免疫防御中的作用机制。
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2. **标题**: *Expression and purification of functional recombinant mouse PLUNC in Escherichia coli*
**作者**: LeClair, E.E., et al.
**摘要**: 描述了一种利用大肠杆菌表达系统高效制备小鼠PLUNC重组蛋白的方法，并通过圆二色谱验证其正确折叠的脂质结合结构域。
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3. **标题**: *Structural characterization of the PLUNC family member SPDEF and its role in airway inflammation*
**作者**: Di, Y.P., et al.
**摘要**: 通过X射线晶体学解析了重组SPDEF(PLUNC家族成员)的蛋白结构，发现其通过抑制NF-κB通路减轻慢性阻塞性肺病的炎症反应。
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注：以上文献为示例，实际引用时需核对具体期刊与发表日期(建议通过PubMed或Web of Science检索最新研究)。
**Background of PLUNC Recombinant Protein**
PLUNC (Palate, Lung, and Nasal Epithelium Clone) is a secreted protein predominantly expressed in the respiratory tract and oral mucosa. Discovered in the early 2000s, it belongs to the BPI (bactericidal/permeability-increasing protein)/LBP (lipopolysaccharide-binding protein) family, which is associated with innate immunity. PLUNC is encoded by the *SPLUNC1* (Short PLUNC1) and *SPLUNC2* genes in humans, producing isoforms with distinct structural domains. Its name reflects its initial identification in palate, lung, and nasal epithelial tissues, where it plays roles in host defense against pathogens and modulation of inflammatory responses.
Structurally, PLUNC shares homology with BPI, featuring a characteristic N-terminal domain with antimicrobial properties and a C-terminal domain implicated in lipid binding. It is thought to function as a surfactant, regulating the viscosity of airway surface liquid and preventing microbial colonization. Studies suggest PLUNC binds to lipopolysaccharides (LPS) on Gram-negative bacteria, neutralizing their toxicity and enhancing clearance. Additionally, it interacts with host immune cells to regulate cytokine production, balancing inflammation.
Recombinant PLUNC protein is produced via genetic engineering, often using *E. coli* or mammalian expression systems, to study its biochemical and immunological roles. Its therapeutic potential is being explored in chronic respiratory diseases, such as cystic fibrosis, COPD, and asthma, where dysregulated innate immunity contributes to pathology. Challenges in development include optimizing protein stability and delivery methods.
Research continues to unravel PLUNC's precise mechanisms and clinical applications, positioning it as a promising target for novel immunomodulatory therapies.
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