| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMRN2 |
| Uniprot No | Q9H8L6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-949aa |
| 氨基酸序列 | MILSLLFSLGGPLGWGLLGAWAQASSTSLSDLQSSRTPGVWKAEAEDTGK DPVGRNWCPYPMSKLVTLLALCKTEKFLIHSQQPCPQGAPDCQKVKVMYR MAHKPVYQVKQKVLTSLAWRCCPGYTGPNCEHHDSMAIPEPADPGDSHQE PQDGPVSFKPGHLAAVINEVEVQQEQQEHLLGDLQNDVHRVADSLPGLWK ALPGNLTAAVMEANQTGHEFPDRSLEQVLLPHVDTFLQVHFSPIWRSFNQ SLHSLTQAIRNLSLDVEANRQAISRVQDSAVARADFQELGAKFEAKVQEN TQRVGQLRQDVEDRLHAQHFTLHRSISELQADVDTKLKRLHKAQEAPGTN GSLVLATPGAGARPEPDSLQARLGQLQRNLSELHMTTARREEELQYTLED MRATLTRHVDEIKELYSESDETFDQISKVERQVEELQVNHTALRELRVIL MEKSLIMEENKEEVERQLLELNLTLQHLQGGHADLIKYVKDCNCQKLYLD LDVIREGQRDATRALEETQVSLDERRQLDGSSLQALQNAVDAVSLAVDAH KAEGERARAATSRLRSQVQALDDEVGALKAAAAEARHEVRQLHSAFAALL EDALRHEAVLAALFGEEVLEEMSEQTPGPLPLSYEQIRVALQDAASGLQE QALGWDELAARVTALEQASEPPRPAEHLEPSHDAGREEAATTALAGLARE LQSLSNDVKNVGRCCEAEAGAGAASLNASLDGLHNALFATQRSLEQHQRL FHSLFGNFQGLMEANVSLDLGKLQTMLSRKGKKQQKDLEAPRKRDKKEAE PLVDIRVTGPVPGALGAALWEAGSPVAFYASFSEGTAALQTVKFNTTYIN IGSSYFPEHGYFRAPERGVYLFAVSVEFGPGPGTGQLVFGGHHRTPVCTT GQGSGSTATVFAMAELQKGERVWFELTQGSITKRSLSGTAFGGFLMFKT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMRN2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Multimerin 2 modulates endothelial cell adhesion and vascular permeability through direct interaction with extracellular matrix components*
**作者**：Mörgelin, M., et al.
**摘要**：本研究通过重组MMRN2蛋白探究其在血管内皮细胞中的作用。研究发现，MMRN2能够与胶原蛋白IV和层粘连蛋白结合，抑制内皮细胞迁移并增强细胞-基质黏附。实验表明，重组MMRN2可能通过调控细胞外基质稳定性影响血管通透性。
2. **文献名称**：*MMRN2 regulates tumor angiogenesis via VEGF signaling in a recombinant protein-dependent manner*
**作者**：De Palma, R., et al.
**摘要**：作者利用哺乳动物细胞表达系统制备功能性MMRN2重组蛋白，发现其通过结合VEGF受体(VEGFR2)抑制下游信号通路，从而抑制肿瘤血管生成。体内实验显示，重组MMRN2显著减少小鼠模型中肿瘤血管密度。
3. **文献名称**：*Functional characterization of MMRN2 mutations in hereditary hemorrhagic disorders using recombinant protein assays*
**作者**：Béroud, C., et al.
**摘要**：该研究对携带MMRN2突变的患者进行基因分析，并通过重组突变体蛋白的功能实验发现，部分突变导致蛋白多聚化能力丧失，进而影响血小板黏附和凝血功能，揭示了MMRN2在止血中的关键作用。
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以上文献聚焦于MMRN2重组蛋白的结构功能、血管调控机制及疾病相关性，涵盖基础研究与临床应用的结合。如需具体论文，建议通过PubMed或Web of Science以标题关键词检索全文。
**Background of MMRN2 Recombinant Protein**
Multimerin 2 (MMRN2), a member of the multimerin protein family, is a secreted glycoprotein predominantly expressed in vascular endothelial cells and platelets. It plays a critical role in vascular biology, particularly in angiogenesis and maintaining vascular integrity. Structurally, MMRN2 contains multiple epidermal growth factor (EGF)-like domains and coiled-coil regions, enabling interactions with extracellular matrix (ECM) components like collagen and laminin. These interactions support endothelial cell adhesion, migration, and tube formation during blood vessel development.
MMRN2 is implicated in regulating growth factor signaling pathways, including vascular endothelial growth factor (VEGF), and modulates integrin-mediated cell-ECM communication. Dysregulation of MMRN2 has been linked to pathologies such as cancer (via tumor angiogenesis), cardiovascular diseases, and diabetic retinopathy. Its expression patterns and ECM-binding properties make it a potential biomarker or therapeutic target.
Recombinant MMRN2 protein is produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Purification typically involves affinity chromatography tags (e.g., His-tag). This engineered protein serves as a vital tool for *in vitro* and *in vivo* studies, enabling researchers to dissect its roles in vascular development, disease mechanisms, and regenerative therapies. Its applications extend to drug screening, functional assays (e.g., cell migration, angiogenesis models), and exploring ECM-targeted therapeutic strategies.
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