| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REPIN1 |
| Uniprot No | Q9BWE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MLERRCRGPL AMGLAQPRLL SGPSQESPQT LGKESRGLRQ QGTSVAQSGA QAPGRAHRCA HCRRHFPGWV ALWLHTRRCQ ARLPLPCPEC GRRFRHAPFL ALHRQVHAAA TPDLGFACHL CGQSFRGWVA LVLHLRAHSA AKRPIACPKC ERRFWRRKQL RAHLRRCHPP APEARPFICG NCGRSFAQWD QLVAHKRVHV AEALEEAAAK ALGPRPRGRP AVTAPRPGGD AVDRPFQCAC CGKRFRHKPN LIAHRRVHTG ERPHQCPECG KRFTNKPYLT SHRRIHTGEK PYPCKECGRR FRHKPNLLSH SKIHKRSEGS AQAAPGPGSP QLPAGPQESA AEPTPAVPLK PAQEPPPGAP PEHPQDPIEA PPSLYSCDDC GRSFRLERFL RAHQRQHTGE RPFTCAECGK NFGKKTHLVA HSRVHSGERP FACEECGRRF SQGSHLAAHR RDHAPDRPFV CPDCGKAFRH KPYLAAHRRI HTGEKPYVCP DCGKAFSQKS NLVSHRRIHT GERPYACPDC DRSFSQKSNL ITHRKSHIRD GAFCCAICGQ TFDDEERLLA HQKKHDV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REPIN1重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: *REPIN1 regulates lipid metabolism and adipocyte differentiation through transcriptional control of PPARγ*
**作者**: Büchler, C., et al.
**摘要**: 本研究利用重组REPIN1蛋白探讨其在脂肪细胞分化中的作用。发现REPIN1通过结合PPARγ基因启动子区域，增强其转录活性，促进脂肪生成和脂滴积累，提示其在肥胖相关代谢疾病中的潜在调控机制。
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2. **文献名称**: *Recombinant REPIN1 modulates hepatic steatosis by interacting with FABP4 in vitro*
**作者**: Ding, Y., et al.
**摘要**: 通过体外表达纯化REPIN1重组蛋白，研究发现其与脂肪酸结合蛋白FABP4直接互作，调节肝细胞内的脂质代谢通路，抑制REPIN1可减少高脂诱导的肝脏脂肪变性，为治疗非酒精性脂肪肝提供新靶点。
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3. **文献名称**: *Structural and functional analysis of REPIN1 zinc finger domains using recombinant protein mutants*
**作者**: Sakai, J., et al.
**摘要**: 本研究通过构建REPIN1重组蛋白的锌指结构域突变体，结合X射线晶体学分析，揭示了其特异性DNA结合模式。功能实验表明，锌指结构破坏会显著降低REPIN1对靶基因的调控能力，阐明其分子作用机制。
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**备注**：上述文献信息为示例性质，实际引用时建议通过PubMed或Web of Science等数据库核对最新研究。
REPIN1 (Replication Initiator Protein 1) is a zinc finger-containing protein encoded by the *REPIN1* gene, primarily recognized for its role in regulating DNA replication initiation and chromatin organization. It binds to specific genomic regions to facilitate the assembly of replication machinery, ensuring precise duplication of genetic material during cell division. Beyond its replication-related functions, REPIN1 has been implicated in metabolic regulation, particularly in adipogenesis and lipid metabolism. Studies suggest its involvement in obesity and metabolic syndrome, as murine models with *REPIN1* overexpression exhibit increased adipose tissue mass and insulin resistance. These findings position REPIN1 as a potential therapeutic target for metabolic disorders.
Recombinant REPIN1 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. The purified protein retains DNA-binding activity and is utilized to investigate its molecular interactions, such as binding to replication origins or transcriptional co-regulators. Researchers also employ it to dissect its role in epigenetic modulation and chromatin remodeling. Additionally, recombinant REPIN1 serves as a tool for screening small-molecule inhibitors aimed at modulating its activity in metabolic diseases. Despite progress, its pleiotropic effects in cellular processes necessitate further exploration to clarify context-dependent mechanisms and therapeutic applicability. Current research continues to unravel its dual roles in genome stability and metabolic homeostasis, bridging gaps between DNA replication biology and systemic energy regulation.
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